Overview

  • Product name
  • Description
    Rabbit polyclonal to ATG4B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Recombinant fragment within Human ATG4B (internal sequence). The exact sequence is proprietary.
    Database link: Q9Y4P1

  • Positive control
    • WB: HepG2 whole cell lysate. IHC-P: Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function
    Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
  • Tissue specificity
    Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
  • Sequence similarities
    Belongs to the peptidase C54 family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • APG4 autophagy 4 homolog B antibody
    • APG4B antibody
    • ATG4 autophagy related 4 homolog B (S. cerevisiae) antibody
    • ATG4B antibody
    • ATG4B_HUMAN antibody
    • AUT like 1 cysteine endopeptidase antibody
    • AUT-like 1 cysteine endopeptidase antibody
    • AUTL1 antibody
    • Autophagin 1 antibody
    • Autophagin-1 antibody
    • Autophagy related 4B cysteine peptidase antibody
    • Autophagy related cysteine endopeptidase 1 antibody
    • Autophagy related protein 4 homolog B antibody
    • Autophagy-related cysteine endopeptidase 1 antibody
    • Autophagy-related protein 4 homolog B antibody
    • Cysteine protease ATG4B antibody
    • hAPG4B antibody
    • MGC1353 antibody
    see all

Images

  • Anti-ATG4B antibody (ab227544) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 44 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded human colon carcinoma tissue stained for ATG4B using ab227544 at 1/500 dilution in immunohistochemical analysis.

References

ab227544 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227544.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up