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Rabbit polyclonal to ATG4B
Predicted to work with:
Mouse, Rat, Pig
Recombinant fragment within Human ATG4B (internal sequence). The exact sequence is proprietary.
Database link: Q9Y4P1
WB: HepG2 whole cell lysate.
IHC-P: Human colon carcinoma tissue.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 44 kDa.
1/100 - 1/1000.
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
Belongs to the peptidase C54 family.
Information by UniProt
APG4 autophagy 4 homolog B antibody
ATG4 autophagy related 4 homolog B (S. cerevisiae) antibody
Western blot - Anti-ATG4B antibody (ab227544)
Anti-ATG4B antibody (ab227544) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size:
10% SDS-PAGE gel.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4B antibody (ab227544)
Paraffin-embedded human colon carcinoma tissue stained for ATG4B using ab227544 at 1/500 dilution in immunohistochemical analysis.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"