Product nameAnti-ATG4B antibody
See all ATG4B primary antibodies
DescriptionRabbit polyclonal to ATG4B
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human ATG4B aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human Heart and Liver Tissue Lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa).|
FunctionCysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Tissue specificityMainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
Sequence similaritiesBelongs to the peptidase C54 family.
- Information by UniProt
- APG4 autophagy 4 homolog B antibody
- APG4B antibody
- ATG4 autophagy related 4 homolog B (S. cerevisiae) antibody
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: ATG4B knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab72878 observed at 44 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab72878 was shown to recognize ATG4B when ATG4B knockout samples were used, along with additional cross-reactive bands. Wild-type and ATG4B knockout samples were subjected to SDS-PAGE. ab72878 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-ATG4B antibody (ab72878) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutes
ab72878 has not yet been referenced specifically in any publications.