Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-ATG4B antibody [EPR6436(2)] (ab154843)

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Overview

  • Product name

    Anti-ATG4B antibody [EPR6436(2)]
    See all ATG4B primary antibodies

  • Description

    Rabbit monoclonal [EPR6436(2)] to ATG4B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human ATG4B. The exact sequence is proprietary.

  • Positive control

    • Recombinant Human ATG4B protein (ab123195) can be used as a positive control in WB. Ramos, 293T, Jurkat and HeLa cell lysates; Jurkat cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.

    • Target

    • Function

      Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

    • Tissue specificity

      Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.

    • Sequence similarities

      Belongs to the peptidase C54 family.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • APG4 autophagy 4 homolog B antibody
      • APG4B antibody
      • ATG4 autophagy related 4 homolog B (S. cerevisiae) antibody
      • ATG4B antibody
      • ATG4B_HUMAN antibody
      • AUT like 1 cysteine endopeptidase antibody
      • AUT-like 1 cysteine endopeptidase antibody
      • AUTL1 antibody
      • Autophagin 1 antibody
      • Autophagin-1 antibody
      • Autophagy related 4B cysteine peptidase antibody
      • Autophagy related cysteine endopeptidase 1 antibody
      • Autophagy related protein 4 homolog B antibody
      • Autophagy-related cysteine endopeptidase 1 antibody
      • Autophagy-related protein 4 homolog B antibody
      • Cysteine protease ATG4B antibody
      • hAPG4B antibody
      • MGC1353 antibody
      see all

    Images

    • Western blot - Anti-ATG4B antibody [EPR6436(2)] (ab154843)
      Western blot - Anti-ATG4B antibody [EPR6436(2)] (ab154843)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: ATG4B knockout HAP1 cell lysate (20 µg)

      Lanes 1 - 2: Merged signal (red and green). Green - ab154843 observed at 47 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab154843 was shown to specifically react with ATG4B when ATG4B knockout samples were used. Wild-type and ATG4B knockout samples were subjected to SDS-PAGE. ab154843 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-ATG4B antibody [EPR6436(2)] (ab154843)
      Western blot - Anti-ATG4B antibody [EPR6436(2)] (ab154843)
      All lanes : Anti-ATG4B antibody [EPR6436(2)] (ab154843) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 44 kDa

    • Flow Cytometry - Anti-ATG4B antibody [EPR6436(2)] (ab154843)
      Flow Cytometry - Anti-ATG4B antibody [EPR6436(2)] (ab154843)
      Flow cytometry analysis of permeabilized Jurkat cells labeling ATG4B (red), using ab154843 at a 1/100 dilution, and negative control cells probed with a Rabbit IgG (green)

    References

    This product has been referenced in:

    • Li Z  et al. Targeting the miR-665-3p-ATG4B-autophagy axis relieves inflammation and apoptosis in intestinal ischemia/reperfusion. Cell Death Dis 9:483 (2018). Read more (PubMed: 29706629) »
    • Li L  et al. Forkhead box O3 (FoxO3) regulates kidney tubular autophagy following urinary tract obstruction. J Biol Chem 292:13774-13783 (2017). Read more (PubMed: 28705935) »
    See all 3 Publications for this product

    Publishing research using ab154843? Please let us know so that we can cite the reference in this datasheet.

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