Key features and details
- Rabbit polyclonal to ATG4C
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ATG4C antibody
See all ATG4C primary antibodies
DescriptionRabbit polyclonal to ATG4C
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- WB: HEK-293T cell lysate. ICC/IF: RKO cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add 1% BSA for extra stability. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
FunctionCysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Tissue specificityHighly expressed in skeletal muscle, heart, liver and testis.
Sequence similaritiesBelongs to the peptidase C54 family.
- Information by UniProt
- APG4 autophagy 4 homolog C (S. cerevisiae) antibody
- APG4 autophagy 4 homolog C antibody
- APG4 C antibody
4% formaldehyde-fixed RKO cells stained for ATG4C (green) using ab223370 at 1/100 dilution in ICC/IF. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain (red) at 1/1000 for 60 minutes at room temperature; DAPI (blue) nuclear stain at 1/5000 for 5 minutes at room temperature.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223370 (D) Merge
Anti-ATG4C antibody (ab223370) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 15 µg
Goat Anti-Rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 6 minutes
Primary antibody was incubated for 16 hours at 4°C, the secondary for 60 minutes at room temperature.
ab223370 has not yet been referenced specifically in any publications.