Key features and details
- Rabbit polyclonal to ATG4C - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ATG4C antibody - C-terminal
See all ATG4C primary antibodies
DescriptionRabbit polyclonal to ATG4C - C-terminal
Specificityab191705 is predicted to not cross-react with other ATG4 proteins.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human ATG4C (C terminal). An 18 amino acid peptide near the carboxy terminus of Human ATG4C. NP_835739.
Database link: Q96DT6
- A549 cell lysate. Human lung tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 52 kDa).|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionCysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Tissue specificityHighly expressed in skeletal muscle, heart, liver and testis.
Sequence similaritiesBelongs to the peptidase C54 family.
- Information by UniProt
- APG4 autophagy 4 homolog C (S. cerevisiae) antibody
- APG4 autophagy 4 homolog C antibody
- APG4 C antibody
Anti-ATG4C antibody - C-terminal (ab191705) at 1 µg/ml + A549 cell lysate at 15 µg
Predicted band size: 52 kDa
Immunofluorescent analysis of human lung tissue labeling ATG4C with ab181391 at 20 µg/ml
Immunohistochemical analysis of human lung tissue labeling ATG4C with ab191705 at 5 µg/ml.
ab191705 has been referenced in 1 publication.
- Liu H et al. Downregulation of DLL4 predicts poor survival in non-small cell lung cancer patients due to promotion of lymph node metastasis. Oncol Rep 40:2988-2996 (2018). PubMed: 30226615