Overview

  • Product name

    Anti-ATG4C antibody - C-terminal
    See all ATG4C primary antibodies
  • Description

    Rabbit polyclonal to ATG4C - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ATG4C (C terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
    Database link: Q96DT6

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab227610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
  • Tissue specificity

    Highly expressed in skeletal muscle, heart, liver and testis.
  • Sequence similarities

    Belongs to the peptidase C54 family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • APG4 autophagy 4 homolog C (S. cerevisiae) antibody
    • APG4 autophagy 4 homolog C antibody
    • APG4 C antibody
    • APG4-C antibody
    • APG4C antibody
    • ATG 4C antibody
    • ATG4 autophagy related 4 homolog C (S. cerevisiae) antibody
    • ATG4 autophagy related 4 homolog C antibody
    • Atg4c antibody
    • ATG4C_HUMAN antibody
    • AUT (S. cerevisiae) like 1, cysteine endopeptidase antibody
    • AUT like 1, cysteine endopeptidase (S. cerevisiae) antibody
    • AUT like 1, cysteine endopeptidase antibody
    • AUT like 3 cysteine endopeptidase antibody
    • AUT-like 3 cysteine endopeptidase antibody
    • AUTL1 antibody
    • AUTL3 antibody
    • Autophagin 3 antibody
    • Autophagin-3 antibody
    • Autophagy related 4C cysteine peptidase antibody
    • Autophagy related cysteine endopeptidase 3 antibody
    • Autophagy related protein 4 homolog C antibody
    • Autophagy-related cysteine endopeptidase 3 antibody
    • Autophagy-related protein 4 homolog C antibody
    • Cysteine protease ATG4C antibody
    • EC 3.4.22 antibody
    • FLJ14867 antibody
    • OTTHUMP00000010715 antibody
    see all

Images

  • All lanes : Anti-ATG4C antibody - C-terminal (ab227610) at 1/1000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lanes 2-3 : HEK-293T transfected with ATG4C shRNA, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 52 kDa



    10% SDS-PAGE

  • All lanes : Anti-ATG4C antibody - C-terminal (ab227610) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 52 kDa



    10% SDS-PAGE

  • ATG4C was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen whole cell lysate with 5 μg ab227610. Western blot was performed from the immunoprecipitate using ab227610. Anti-Rabbit IgG was used as secondary antibody.

    Lane 1: HEK-293T whole cell extract (Input).

    Lane 2: Control IgG IP in HEK-293T whole cell extract.

    Lane 3: ab227610 IP in HEK-293T whole cell extract.

References

ab227610 has not yet been referenced specifically in any publications.

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