Overview

  • Product name

  • Description

    Rabbit polyclonal to ATG4D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 220-441 of Human ATG4D.

  • Positive control

    • A431, H1299, HeLa and HepG2 cell lysates; SAS xenograft tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab137621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 53 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
  • Tissue specificity

    Mainly expressed in skeletal muscle and, to a lower extent, in testis.
  • Sequence similarities

    Belongs to the peptidase C54 family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 9830134P12Rik antibody
    • APG4 autophagy 4 homolog D antibody
    • APG4 D antibody
    • APG4, S. cerevisiae, homolog of, D antibody
    • APG4D antibody
    • ATG4 autophagy related 4 homolog D (S. cerevisiae) antibody
    • Atg4d antibody
    • ATG4D_HUMAN antibody
    • Atg4dl antibody
    • AUT like 4 cysteine endopeptidase antibody
    • AUT-like 4 cysteine endopeptidase antibody
    • AUTL4 antibody
    • Autophagin 4 antibody
    • Autophagin-4 antibody
    • autophagy 4, S. cerevisiae, homolog of, D antibody
    • autophagy related 4D, cysteine peptidase antibody
    • Autophagy related cysteine endopeptidase 4 antibody
    • Autophagy related protein 4 homolog D antibody
    • Autophagy-related cysteine endopeptidase 4 antibody
    • Autophagy-related protein 4 homolog D antibody
    • Cysteine protease ATG4D antibody
    • Cysteine protease involved in autophagy antibody
    • MGC31226 antibody
    see all

Images

  • Anti-ATG4D antibody (ab137621) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 53 kDa



    10% SDS PAGE
  • ab137621 at a 1/100 dilution, staining ATG4D in paraffin embedded SAS xenograft tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Andaloussi AE  et al. Defective expression of ATG4D abrogates autophagy and promotes growth in human uterine fibroids. Cell Death Discov 3:17041 (2017). Read more (PubMed: 28815060) »
  • Zhang J  et al. MECP2 expression in gastric cancer and its correlation with clinical pathological parameters. Medicine (Baltimore) 96:e7691 (2017). WB, IHC ; Human . Read more (PubMed: 28767600) »
See all 4 Publications for this product

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