Overview

  • Product name

  • Description

    Rabbit polyclonal to ATG4D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human ATG4D aa 14-43.
    Database link: Q86TL0

  • Positive control

    • WB: A549, HeLa and SH-SY5Y whole cell lysates; Mouse heart tissue lysate. IHC-P: Human small intestine and pancreatic tissues. ICC/IF: PC-3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab237751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 53 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/500.

Target

  • Function

    Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
  • Tissue specificity

    Mainly expressed in skeletal muscle and, to a lower extent, in testis.
  • Sequence similarities

    Belongs to the peptidase C54 family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 9830134P12Rik antibody
    • APG4 autophagy 4 homolog D antibody
    • APG4 D antibody
    • APG4, S. cerevisiae, homolog of, D antibody
    • APG4D antibody
    • ATG4 autophagy related 4 homolog D (S. cerevisiae) antibody
    • Atg4d antibody
    • ATG4D_HUMAN antibody
    • Atg4dl antibody
    • AUT like 4 cysteine endopeptidase antibody
    • AUT-like 4 cysteine endopeptidase antibody
    • AUTL4 antibody
    • Autophagin 4 antibody
    • Autophagin-4 antibody
    • autophagy 4, S. cerevisiae, homolog of, D antibody
    • autophagy related 4D, cysteine peptidase antibody
    • Autophagy related cysteine endopeptidase 4 antibody
    • Autophagy related protein 4 homolog D antibody
    • Autophagy-related cysteine endopeptidase 4 antibody
    • Autophagy-related protein 4 homolog D antibody
    • Cysteine protease ATG4D antibody
    • Cysteine protease involved in autophagy antibody
    • MGC31226 antibody
    see all

Images

  • All lanes : Anti-ATG4D antibody (ab237751) at 1/500 dilution

    Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 4 : Mouse heart tissue lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 53 kDa

  • Paraffin-embedded human small intestine tissue stained for ATG4D using ab237751 at 1/700 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

  • Paraffin-embedded human pancreatic tissue stained for ATG4D using ab237751 at 1/700 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

  • PC-3 (Human prostate adenocarcinoma cell line) cells stained for ATG4D (green) using ab237751 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor®488 conjugated Goat Anti-Rabbit IgG (H+L).

References

ab237751 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab237751.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up