Product nameAnti-ATG7 antibody
See all ATG7 primary antibodies
DescriptionRabbit polyclonal to ATG7
Tested applicationsSuitable for: IHC-FoFr, ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Chicken, Human
Synthetic peptide corresponding to Human ATG7 (C terminal).
- Caco-2 cell lysate, MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab53255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 21784844|
|ICC/IF||Use a concentration of 5 - 10 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).|
|IHC-P||Use at an assay dependent concentration. PubMed: 20174468|
FunctionE1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.
Tissue specificityWidely expressed, especially in kidney, liver, lymph nodes and bone marrow.
Sequence similaritiesBelongs to the ATG7 family.
DomainThe C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.
The N-terminal FAP motif (residues 15 to 17) is essential for the formation of the ATG89-PE and ATG5-ATG12 conjugates.
modificationsAcetylated by EP300.
Cellular localizationCytoplasm. Preautophagosomal structure. Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
- Information by UniProt
- 1810013K23Rik antibody
- Apg 7 antibody
- APG7 autophagy 7 like antibody
Lane 1 : Anti-ATG7 antibody (ab53255) at 0.5 µg/ml
Lane 2 : Anti-ATG7 antibody (ab53255) at 1 µg/ml
Lane 3 : Anti-ATG7 antibody (ab53255) at 2 µg/ml
All lanes : Caco-2 cell lysate at 15 µg per lane.
Predicted band size: 78 kDa
Observed band size: 78 kDa
This image shows ab53255 staining MCF7 cells as 10µg/ml
IHC image of ab53255 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53255, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemical analysis of murine brain tissue, staining ATG7 with ab53255. Fixed brain sections were blocked with serum, permeabilized with 0.2% Triton X-100, and treated with primary antibody. An AlexaFluor®555-conjugated anti-rabbit IgG was used as the secondary antibody.
This product has been referenced in:
- D'Ignazio L et al. Lhx8 ablation leads to massive autophagy of mouse oocytes associated with DNA damage. Biol Reprod 98:532-542 (2018). Read more (PubMed: 29329412) »
- Chen L et al. Juglanin inhibits lung cancer by regulation of apoptosis, ROS and autophagy induction. Oncotarget 8:93878-93898 (2017). Read more (PubMed: 29212196) »