Key features and details
- Rabbit polyclonal to ATG7
- Reacts with: Arabidopsis thaliana
- Isotype: IgG
Product nameAnti-ATG7 antibody
See all ATG7 primary antibodies
DescriptionRabbit polyclonal to ATG7
Species reactivityReacts with: Arabidopsis thaliana
Full length protein corresponding to ATG7.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: Whole serum
Concentration information loading...
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
FunctionE1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.
Tissue specificityWidely expressed, especially in kidney, liver, lymph nodes and bone marrow.
Sequence similaritiesBelongs to the ATG7 family.
DomainThe C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.
The N-terminal FAP motif (residues 15 to 17) is essential for the formation of the ATG89-PE and ATG5-ATG12 conjugates.
modificationsAcetylated by EP300.
Cellular localizationCytoplasm. Preautophagosomal structure. Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
- Information by UniProt
- 1810013K23Rik antibody
- Apg 7 antibody
- APG7 autophagy 7 like antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab99001 has been referenced in 2 publications.
- Qi H et al. Arabidopsis SINAT Proteins Control Autophagy by Mediating Ubiquitylation and Degradation of ATG13. Plant Cell 32:263-284 (2020). PubMed: 31732704
- Yan Q et al. Endocytosis of AtRGS1 Is Regulated by the Autophagy Pathway after D-Glucose Stimulation. Front Plant Sci 8:1229 (2017). PubMed: 28747924