Key features and details
- Rabbit polyclonal to ATG9A - C-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ATG9A antibody - C-terminal
See all ATG9A primary antibodies
DescriptionRabbit polyclonal to ATG9A - C-terminal
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide corresponding to Human ATG9A (C terminal). (18 amino acid peptide).
Database link: Q7Z3C6
- Human spleen and brain (cerebellum) tissues; Human heart tissue; Mouse heart tissue lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab219154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 94 kDa.|
FunctionInvolved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.
Sequence similaritiesBelongs to the ATG9 family.
Cellular localizationCytoplasmic vesicle, autophagosome membrane. Golgi apparatus, trans-Golgi network membrane. Late endosome membrane. Endoplasmic reticulum membrane. Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation-induced redistribution depends on ULK1, ATG13, as well as SH3GLB1.
- Information by UniProt
- APG9 autophagy 9-like 1 antibody
- APG9 like 1 antibody
- APG9-like 1 antibody
Anti-ATG9A antibody - C-terminal (ab219154) at 1 µg/ml + Mouse heart tissue lysate
Predicted band size: 94 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labeling ATG9A with ab219154 at 5 µg/ml.
Immunofluorescent analysis of human heart tissue labeling ATG9A with ab219154 at 20 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain (cerebellum) tissue labeling ATG9A with ab219154 at 5 µg/ml.
ab219154 has not yet been referenced specifically in any publications.