Overview

  • Product name

    Anti-ATG9A antibody - C-terminal
    See all ATG9A primary antibodies
  • Description

    Rabbit polyclonal to ATG9A - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig, Rhesus monkey
  • Immunogen

    Synthetic peptide within Human ATG9A (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
    Database link: Q7Z3C6

  • Positive control

    • WB: HeLa, HEK-293T, A431, PC-12, Rat-2, BCL-1 and RD whole cell lysates. IHC-P: Colon carcinoma tissue. ICC/IF: A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab229334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 94 kDa.
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.

Target

  • Function

    Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.
  • Sequence similarities

    Belongs to the ATG9 family.
  • Cellular localization

    Cytoplasmic vesicle, autophagosome membrane. Golgi apparatus, trans-Golgi network membrane. Late endosome membrane. Endoplasmic reticulum membrane. Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation-induced redistribution depends on ULK1, ATG13, as well as SH3GLB1.
  • Information by UniProt
  • Database links

  • Alternative names

    • APG9 autophagy 9-like 1 antibody
    • APG9 like 1 antibody
    • APG9-like 1 antibody
    • APG9L1 antibody
    • ATG9 antibody
    • ATG9 autophagy related 9 homolog A antibody
    • ATG9 autophagy related 9 homolog A (S. cerevisiae) antibody
    • ATG9A antibody
    • ATG9A_HUMAN antibody
    • Autophagy 9-like 1 protein antibody
    • Autophagy related 9A antibody
    • Autophagy related protein 9A antibody
    • Autophagy-related protein 9A antibody
    • mATG9 antibody
    • MGD3208 antibody
    • OTTHUMP00000206046 antibody
    • OTTHUMP00000206048 antibody
    • OTTHUMP00000206049 antibody
    • OTTHUMP00000206062 antibody
    see all

Images

  • Anti-ATG9A antibody - C-terminal (ab229334) at 1/1000 dilution + RD (human rhabdomyosarcoma cell line) whole cell extract at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 94 kDa



    7.5% SDS-PAGE gel.

  • A431 (human epidermoid carcinoma cell line) cells were stained for ATG9A (green) using ab229334 at 1/500 dilution in ICC/IF. Cells were fixed in 100% methanol for 5 minutes.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Paraffin-embedded colon carcinoma tissue stained for ATG9A using ab229334 at 1/500 dilution in immunohistochemical analysis.

  • Anti-ATG9A antibody - C-terminal (ab229334) at 1/1000 dilution + BCL-1 (mouse B lymphocyte lymphoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 94 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-ATG9A antibody - C-terminal (ab229334) at 1/1000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 94 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-ATG9A antibody - C-terminal (ab229334) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 94 kDa



    7.5% SDS-PAGE gel.

  • ATG9A was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 4 μg of ab229334. Western blot was performed from the immunoprecipitate using ab229334 at 1/500 dilution.

    Lane 1: 4 μg preimmune rabbit igG instead of ab229334 in HeLa whole cell extract.
    Lane 2: ab229334 IP in HeLa whole cell extract.

    7.5% SDS-PAGE gel.

References

ab229334 has not yet been referenced specifically in any publications.

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