Application
Western blot
Sample
Cow Cell lysate - whole cell (Lung)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12)
Loading amount
30 µg
Specification
Lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Buffer
Dilution
1/3000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-Rabbit Ig(H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
3 second(s)
Bands
Specific: ~95 kDa Non-specific: ~100 kDa
Positive control
HEK 293 lysate
Additional data
Additional Notes
This antibody detect correct band but the band is low intense than the equal amount of control protein, indicating ATG9A possibly express low in cow/lung tissue.
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Submitted Apr 05 2017