Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse hippocampal lysate)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
Mouse hippocampal lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: Milk (+ Tween 0.05%)
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti-rabbit HRP conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
50 second(s)
Bands
Specific: 100 kDa
Additional data
Additional Notes
Works excellently for mouse tissues, have used this for WB, ICC and IHC purposes.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Davor Ivankovic
Verified customer
Submitted Jul 16 2018