Key features and details
- Rabbit polyclonal to Atherin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Atherin antibody
DescriptionRabbit polyclonal to Atherin
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Horse, Guinea pig, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region within amino acids 488-538 of Human Atherin (NP_612361.1).
- HeLa and 293T whole cell lysates
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab128170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 56 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay play a role in atherogenesis by immobilizing LDL in the atherial wall.
Tissue specificityExpressed in atherosclerotic lesions, not in normal intima.
Sequence similaritiesContains 1 SAM (sterile alpha motif) domain.
Cellular localizationCytoplasm. Secreted.
- Information by UniProt
- Atherin antibody
- SAM domain containing 1 antibody
- SAM domain-containing protein 1 antibody
All lanes : Anti-Atherin antibody (ab128170) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 56 kDa
Exposure time: 3 minutes
Detection of Atherin in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab128170 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab128170 has not yet been referenced specifically in any publications.