Overview

  • Product name

    Anti-Atherin antibody
  • Description

    Rabbit polyclonal to Atherin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Horse, Guinea pig, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 488-538 of Human Atherin (NP_612361.1).

  • Positive control

    • HeLa and 293T whole cell lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 56 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    May play a role in atherogenesis by immobilizing LDL in the atherial wall.
  • Tissue specificity

    Expressed in atherosclerotic lesions, not in normal intima.
  • Sequence similarities

    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization

    Cytoplasm. Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Atherin antibody
    • SAM domain containing 1 antibody
    • SAM domain-containing protein 1 antibody
    • SAMD1 antibody
    • SAMD1_HUMAN antibody
    • Sterile alpha motif domain containing 1 antibody
    • Sterile alpha motif domain-containing protein 1 antibody
    see all

Images

  • All lanes : Anti-Atherin antibody (ab128170) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 56 kDa


    Exposure time: 3 minutes
  • Detection of Atherin in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab128170 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab128170 has not yet been referenced specifically in any publications.

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