Overview

  • Product name

  • Description

    Rabbit polyclonal to ATIC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human ATIC.
    Database link: P31939

  • Positive control

    • Extracts from MCF7, SW480, H460, HepG2, SW620, HT29 and K562 cell lines.

Properties

Applications

Our Abpromise guarantee covers the use of ab186725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/2000. Predicted molecular weight: 65 kDa.
IHC-P 1/50 - 1/200.

Target

  • Function

    Bifunctional enzyme that catalyzes 2 steps in purine biosynthesis.
  • Pathway

    Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.
    Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
  • Involvement in disease

    Defects in ATIC are the cause of AICA-ribosuria [MIM:608688]; also known as AICA-ribosiduria. AICA-ribosuria is a neurologically devastating inborn error of purine biosynthesis. AICA-ribosuria patients excrete massive amounts of AICA-riboside in the urine and accumulate AICA-ribotide and its derivatives in erythrocytes and fibroblasts. AICA-ribosuria causes profound mental retardation, epilepsy, dysmorphic features and congenital blindness.
  • Sequence similarities

    Belongs to the purH family.
  • Domain

    The IMP cyclohydrolase activity resides in the N-terminal region.
  • Information by UniProt
  • Database links

  • Alternative names

    • AICAR formyltransferase/IMP cyclohydrolase antibody
    • 5 aminoimidazole 4 carboxamide 1 beta D ribonucleotide transformylase/inosinicase antibody
    • 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase antibody
    • 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase antibody
    • 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase antibody
    • AICAR antibody
    • AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme antibody
    • AICAR transformylase antibody
    • AICARFT antibody
    • AICARFT/IMPCHASE antibody
    • ATIC antibody
    • Bifunctional purine biosynthesis protein PURH antibody
    • epididymis secretory sperm binding protein Li 70p antibody
    • FLJ93545 antibody
    • HEL-S-70p antibody
    • IMP cyclohydrolase antibody
    • IMP synthase antibody
    • IMP synthetase antibody
    • IMPCHASE antibody
    • Inosinicase antibody
    • OK/SW-cl.86 antibody
    • Phosphoribosylaminoimidazolecarboxamide formyltransferase antibody
    • Phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase antibody
    • PUR9_HUMAN antibody
    • PURH antibody
    see all

Images

  • All lanes : Anti-ATIC antibody (ab186725) at 1/500 dilution

    Lane 1 : Extract from MCF7 cell line.
    Lane 2 : Extract from SW480 cell line.
    Lane 3 : Extract from H460 cell line.
    Lane 4 : Extract from HepG2 cell line.
    Lane 5 : Extract from SW620 cell line.
    Lane 6 : Extract from HT29 cell line.
    Lane 7 : Extract from K562 cell line.

    Predicted band size: 65 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue labelling ATIC with ab186725 at 1/200. Magnification: 400x.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophagus tissue labelling ATIC with ab186725 at 1/200. Magnification: 400x.
  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186725. Blue DAPI for nuclear staining.

References

ab186725 has not yet been referenced specifically in any publications.

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