Key features and details
- Rabbit polyclonal to ATIC
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ATIC antibody
See all ATIC primary antibodies
DescriptionRabbit polyclonal to ATIC
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human ATIC.
Database link: P31939
- Extracts from MCF7, SW480, H460, HepG2, SW620, HT29 and K562 cell lines.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab186725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/2000. Predicted molecular weight: 65 kDa.|
|IHC-P||1/50 - 1/200.|
FunctionBifunctional enzyme that catalyzes 2 steps in purine biosynthesis.
PathwayPurine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.
Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Involvement in diseaseDefects in ATIC are the cause of AICA-ribosuria [MIM:608688]; also known as AICA-ribosiduria. AICA-ribosuria is a neurologically devastating inborn error of purine biosynthesis. AICA-ribosuria patients excrete massive amounts of AICA-riboside in the urine and accumulate AICA-ribotide and its derivatives in erythrocytes and fibroblasts. AICA-ribosuria causes profound mental retardation, epilepsy, dysmorphic features and congenital blindness.
Sequence similaritiesBelongs to the purH family.
DomainThe IMP cyclohydrolase activity resides in the N-terminal region.
- Information by UniProt
- AICAR formyltransferase/IMP cyclohydrolase antibody
- 5 aminoimidazole 4 carboxamide 1 beta D ribonucleotide transformylase/inosinicase antibody
- 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase antibody
All lanes : Anti-ATIC antibody (ab186725) at 1/500 dilution
Lane 1 : Extract from MCF7 cell line.
Lane 2 : Extract from SW480 cell line.
Lane 3 : Extract from H460 cell line.
Lane 4 : Extract from HepG2 cell line.
Lane 5 : Extract from SW620 cell line.
Lane 6 : Extract from HT29 cell line.
Lane 7 : Extract from K562 cell line.
Predicted band size: 65 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue labelling ATIC with ab186725 at 1/200. Magnification: 400x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophagus tissue labelling ATIC with ab186725 at 1/200. Magnification: 400x.
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186725. Blue DAPI for nuclear staining.
ab186725 has not yet been referenced specifically in any publications.