Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13243-53] to ATIC
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Human
Product nameAnti-ATIC antibody [EPR13243-53]
See all ATIC primary antibodies
DescriptionRabbit monoclonal [EPR13243-53] to ATIC
Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
Species reactivityReacts with: Human
Recombinant fragment within Human ATIC aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: P31939
- Jurkat, HeLa, HepG2 and HCT-116 cell lysates; HeLa cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab188321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/40 - 1/60.|
FunctionBifunctional enzyme that catalyzes 2 steps in purine biosynthesis.
PathwayPurine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.
Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Involvement in diseaseDefects in ATIC are the cause of AICA-ribosuria [MIM:608688]; also known as AICA-ribosiduria. AICA-ribosuria is a neurologically devastating inborn error of purine biosynthesis. AICA-ribosuria patients excrete massive amounts of AICA-riboside in the urine and accumulate AICA-ribotide and its derivatives in erythrocytes and fibroblasts. AICA-ribosuria causes profound mental retardation, epilepsy, dysmorphic features and congenital blindness.
Sequence similaritiesBelongs to the purH family.
DomainThe IMP cyclohydrolase activity resides in the N-terminal region.
- Information by UniProt
- AICAR formyltransferase/IMP cyclohydrolase antibody
- 5 aminoimidazole 4 carboxamide 1 beta D ribonucleotide transformylase/inosinicase antibody
- 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase antibody
All lanes : Anti-ATIC antibody [EPR13243-53] (ab188321) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HCT-116 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling ATIC with ab188321 at 1/140 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Western blot analysis of ATIC in Jurkat cell lysate immunoprecipitated with ab188321 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Jurkat lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
ab188321 has been referenced in 1 publication.
- Wang H et al. Deletion of OSBPL2 in auditory cells increases cholesterol biosynthesis and drives reactive oxygen species production by inhibiting AMPK activity. Cell Death Dis 10:627 (2019). PubMed: 31427568