Key features and details
- Rabbit polyclonal to ATL3
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ATL3 antibody
See all ATL3 primary antibodies
DescriptionRabbit polyclonal to ATL3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide, corresponding to a region within amino acids 350-400 of Human ATL3 according to NP_056274.3.
- Whole cell lysate from HeLa, Jurkat, 293T and mouse NIH3T3 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab117819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 61 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionGTPase tethering membranes through formation of trans-homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis.
Tissue specificityExpressed in peripheral tissues (at protein level).
Sequence similaritiesBelongs to the GBP family. Atlastin subfamily.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- 4633402C03Rik antibody
- 5730596K20Rik antibody
- AI465397 antibody
All lanes : Anti-ATL3 antibody (ab117819) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Exposure time: 30 seconds
Detection of Human ATL3 by Western Blot of Immunoprecipitation.
ab117819 at 1µg/ml staining ATL3 in HeLa whole cell lysate immunoprecipitated using ab117819 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
ab117819 has not yet been referenced specifically in any publications.