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You mention in the datasheet and the figures that HEK293 cell lysate treated with doxorubicin can be used as a positive control.
Which dose and which duration of treatment do you propose?
Asked on Jan 07 2016
I can confirm that HEK293 cells were treated with 10uM doxorubicin for 24 hours. After the treatment, the cells were prepared into lysates for WB as normal.
We offer Doxorubicin in two sizes, 10 and 50 mg:
ab120629 (Doxorubicin hydrochloride)
https://www.abcam.com/Doxorubicin-hydrochloride-Topoisomerase-II-inhibitor-ab120629.html (or use the following: https://www.abcam.com/Doxorubicin-hydrochloride-Topoisomerase-II-inhibitor-ab120629.html).
Anja HoffmannAbcam Scientific Support
Answered on Jan 07 2016