Key features and details
- Rabbit polyclonal to ATMIN - N-terminal
- Suitable for: ICC/IF, ICC, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ATMIN antibody - N-terminal
See all ATMIN primary antibodies
DescriptionRabbit polyclonal to ATMIN - N-terminal
Tested applicationsSuitable for: ICC/IF, ICC, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human ATMIN (N terminal). (19 amino acid peptide from near the amino terminus; NP_056066).
Database link: O43313
- WB: 293 cell lysate. ICC/IF: A431 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 2.5 µg/ml.|
|ICC||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 88, 72 kDa).|
FunctionPlays a crucial role in cell survival and RAD51 foci formation in response to methylating DNA damage. Involved in regulating the activity of ATM in the absence of DNA damage. May play a role in stabilizing ATM.
Tissue specificityUbiquitously expressed in normal tissues and cancer cell lines with highest levels in placenta and skeletal muscle.
Sequence similaritiesContains 2 C2H2-type zinc fingers.
Cellular localizationNucleus. Nuclear, in discrete foci during G1 phase.
- Information by UniProt
- ASCIZ antibody
- ATM INteracting protein antibody
- ATM interactor antibody
Lane 1 : Anti-ATMIN antibody - N-terminal (ab191856) at 1 µg/ml
Lane 2 : Anti-ATMIN antibody - N-terminal (ab191856) at 2 µg/ml
All lanes : 293 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 88, 72 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Immunofluorescent analysis of A431 cells labeling ATMIN with ab191856 at 2.5 μg/ml.
Immunocytochemical analysis of A431 cells labeling ATMIN with ab191856 at 5 μg/ml.
ab191856 has not yet been referenced specifically in any publications.