Anti-ATP-binding cassette sub-family A member 3 antibody [3C9] (ab24751)

Overview

  • Product name
    Anti-ATP-binding cassette sub-family A member 3 antibody [3C9]
    See all ATP-binding cassette sub-family A member 3 primary antibodies
  • Description
    Mouse monoclonal [3C9] to ATP-binding cassette sub-family A member 3
  • Host species
    Mouse
  • Specificity
    ab24751 recognises P180 Lamellar Body Protein.
  • Tested applications
    Suitable for: IHC-FoFr, ICC, IHC-P, IHC-Fr, ICC/IF, Electron Microscopy, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Tissue, cells or virus corresponding to Rat ATP-binding cassette sub-family A member 3.

  • Positive control
    • IHC-P: FFPE rat lung tissue sections.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Monoclonal
  • Clone number
    3C9
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 28947744
ICC Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Electron Microscopy Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 191 kDa.

Target

  • Function
    Plays an important role in the formation of pulmonary surfactant, probably by transporting lipids such as cholesterol.
  • Tissue specificity
    Highly expressed in lung, followed by brain, pancreas, skeletal muscle and heart. Weakly expressed in placenta, kidney and liver. Also expressed in medullary thyroid carcinoma cells (MTC) and in C-cell carcinoma.
  • Involvement in disease
    Defects in ABCA3 are the cause of pulmonary surfactant metabolism dysfunction type 3 (SMDP3) [MIM:610921]; also called pulmonary alveolar proteinosis due to ABCA3 deficiency. A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCA family.
    Contains 2 ABC transporter domains.
  • Domain
    Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC 3 antibody
    • ABC C antibody
    • ABC C transporter antibody
    • ABC transporter 3 antibody
    • ABC-C transporter antibody
    • ABC3 antibody
    • ABCA 3 antibody
    • Abca3 antibody
    • ABCA3 protein antibody
    • ABCA3_HUMAN antibody
    • ABCC antibody
    • ATP binding cassette 3 antibody
    • ATP binding cassette sub family A (ABC1) member 3 antibody
    • ATP binding cassette sub family A member 3 antibody
    • ATP binding cassette transporter 3 antibody
    • ATP-binding cassette 3 antibody
    • ATP-binding cassette sub-family A member 3 antibody
    • ATP-binding cassette transporter 3 antibody
    • CED7. C. elegans, homolog of antibody
    • EST111653 antibody
    • LBM 180 antibody
    • LBM180 antibody
    • MGC72201 antibody
    • P180 Lamellar Body Protein antibody
    • SMDP3 antibody
    see all

Images

  • IHC image of P180 Lamellar Body Protein staining in rat lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24751, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Haider C  et al. Effects of ß-Carotene and Its Cleavage Products in Primary Pneumocyte Type II Cells. Antioxidants (Basel) 6:N/A (2017). Read more (PubMed: 28531132) »
  • Pan B  et al. SOX4 arrests lung development in rats with hyperoxia-induced bronchopulmonary dysplasia by controlling EZH2 expression. Int J Mol Med 40:1691-1698 (2017). Read more (PubMed: 29039454) »
See all 10 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (alveolar type II cells)
Permeabilization
Yes - 0,2% Saponin
Specification
alveolar type II cells
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 29 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (epithelial cells)
Specification
epithelial cells
Blocking step
(agent) for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 27 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (primary lung epithelia cells)
Permeabilization
Yes - Saponin
Specification
primary lung epithelia cells
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 1%
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 09 2015

Application
Western blot
Sample
Rat Cell lysate - whole cell (primary lung epithelia cells)
Gel Running Conditions
Reduced Denaturing (4-12)
Loading amount
10 µg
Specification
primary lung epithelia cells
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 1%

Abcam user community

Verified customer

Submitted Oct 09 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary alveolar type II epithelial cells)
Specification
Primary alveolar type II epithelial cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2 saponin
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 30 2012

Answer

Thank you for your telephone enquiry yesterday. Unfortunately, the epitope for this antibody (ab24751) has not been mapped, so it is not known if it is on the outer membrane or on the intracellular section of the protein. I can suggest trying permeabilisation. This should be with a more gentle reagent. Tween 20, Saponin, Digitonin and Leucoperm are mild membrane solubilisers. Use at 0.5% in PBS. These give large enough pores for antibodies to go through without dissolving plasma membrane Harsh detergents such as triton or acetone are not suitable for membrane proteins. We are always pleased to hear from customers who have used antibodies in untested applications. If you decide to go ahead and purchase this product, we would be pleased if you can consider submitting some feedback by submitting an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

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Answer

Thank you for your enquiry. The antibody was tested on a 5-15% gradient gel using reducing conditions. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (lung)
Specification
lung
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1.5

Abcam user community

Verified customer

Submitted Feb 06 2006

Answer

Thank you for your enquiry. This antibody was raised against purified rat lamellar body membranes. We do not know the epitope, as epitope mapping was not done. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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