Key features and details
- Mouse monoclonal [3C9] to ATP-binding cassette sub-family A member 3
- Suitable for: IHC-FoFr, ICC, IHC-P, IHC-Fr, ICC/IF, Electron Microscopy, WB
- Reacts with: Mouse, Rat
- Isotype: IgG2a
Product nameAnti-ATP-binding cassette sub-family A member 3 antibody [3C9]
See all ATP-binding cassette sub-family A member 3 primary antibodies
DescriptionMouse monoclonal [3C9] to ATP-binding cassette sub-family A member 3
Tested applicationsSuitable for: IHC-FoFr, ICC, IHC-P, IHC-Fr, ICC/IF, Electron Microscopy, WBmore details
Species reactivityReacts with: Mouse, Rat
Tissue, cells or virus corresponding to Rat ATP-binding cassette sub-family A member 3.
Database link: P16970
- IHC-P: FFPE rat lung tissue sections.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab24751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 28947744|
|ICC||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|Electron Microscopy||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 191 kDa.|
FunctionPlays an important role in the formation of pulmonary surfactant, probably by transporting lipids such as cholesterol.
Tissue specificityHighly expressed in lung, followed by brain, pancreas, skeletal muscle and heart. Weakly expressed in placenta, kidney and liver. Also expressed in medullary thyroid carcinoma cells (MTC) and in C-cell carcinoma.
Involvement in diseaseDefects in ABCA3 are the cause of pulmonary surfactant metabolism dysfunction type 3 (SMDP3) [MIM:610921]; also called pulmonary alveolar proteinosis due to ABCA3 deficiency. A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCA family.
Contains 2 ABC transporter domains.
DomainMultifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
- Information by UniProt
- ABC 3 antibody
- ABC C antibody
- ABC C transporter antibody
IHC image of P180 Lamellar Body Protein staining in rat lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24751, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab24751 has been referenced in 13 publications.
- Müller MT et al. Interaction of microtubules and actin during the post-fusion phase of exocytosis. Sci Rep 9:11973 (2019). PubMed: 31427591
- Stucki JD et al. Medium throughput breathing human primary cell alveolus-on-chip model. Sci Rep 8:14359 (2018). PubMed: 30254327
- Kook S et al. Gene-edited MLE-15 Cells as a Model for the Hermansky-Pudlak Syndromes. Am J Respir Cell Mol Biol 58:566-574 (2018). PubMed: 29190429
- Haider C et al. Effects of ß-Carotene and Its Cleavage Products in Primary Pneumocyte Type II Cells. Antioxidants (Basel) 6:N/A (2017). PubMed: 28531132
- Pan B et al. SOX4 arrests lung development in rats with hyperoxia-induced bronchopulmonary dysplasia by controlling EZH2 expression. Int J Mol Med 40:1691-1698 (2017). PubMed: 29039454