Anti-ATP citrate lyase antibody (ab157098)
Key features and details
- Rabbit polyclonal to ATP citrate lyase
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ATP citrate lyase antibody
See all ATP citrate lyase primary antibodies -
Description
Rabbit polyclonal to ATP citrate lyase -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Caenorhabditis elegans, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to Human ATP citrate lyase aa 350-450.
Database link: P53396 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 120 kDa.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 120 kDa. |
Target
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Function
ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. -
Sequence similarities
In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
Contains 1 ATP-grasp domain. -
Post-translational
modificationsISGylated.
Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 395373 Chicken
- Entrez Gene: 607852 Dog
- Entrez Gene: 47 Human
- Entrez Gene: 104112 Mouse
- Entrez Gene: 100125957 Pig
- Entrez Gene: 24159 Rat
- Entrez Gene: 708501 Rhesus monkey
- Entrez Gene: 654404 Sheep
see all -
Alternative names
- ACL antibody
- Acly antibody
- ACLY_HUMAN antibody
see all
Images
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All lanes : Anti-ATP citrate lyase antibody (ab157098) at 0.4 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 120 kDa
Exposure time: 30 seconds -
All lanes : Anti-ATP citrate lyase antibody (ab157098) at 0.4 µg/ml
Lane 1 : NIH3T3 whole cell lysate at 50 µg
Lane 2 : NIH3T3 whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 120 kDa
Exposure time: 10 seconds
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab157098 has been referenced in 4 publications.
- Santarsiero A et al. Phenolic Compounds of Red Wine Aglianico del Vulture Modulate the Functional Activity of Macrophages via Inhibition of NF-?B and the Citrate Pathway. Oxid Med Cell Longev 2021:5533793 (2021). PubMed: 34122722
- Santarsiero A et al. New synthesized polyoxygenated diarylheptanoids suppress lipopolysaccharide-induced neuroinflammation. Biochem Biophys Res Commun 529:1117-1123 (2020). PubMed: 32819574
- Santarsiero A et al. Pistacia lentiscus Hydrosol: Untargeted Metabolomic Analysis and Anti-Inflammatory Activity Mediated by NF-?B and the Citrate Pathway. Oxid Med Cell Longev 2020:4264815 (2020). PubMed: 33204395
- Huang C et al. Soy Isoflavones Regulate Lipid Metabolism through an AKT/mTORC1 Pathway in Diet-Induced Obesity (DIO) Male Rats. Molecules 21:N/A (2016). PubMed: 27153053