Overview

  • Product name
    Anti-ATP citrate lyase antibody
    See all ATP citrate lyase primary antibodies
  • Description
    Rabbit polyclonal to ATP citrate lyase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Caenorhabditis elegans, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 350-400 of Human ATP citrate lyase (NP_001087.2).

  • Positive control
    • Jurkat, HeLa, 293T and NIH3T3 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab157098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 120 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
  • Sequence similarities
    In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
    In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
    Contains 1 ATP-grasp domain.
  • Post-translational
    modifications
    ISGylated.
    Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
    Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACL antibody
    • Acly antibody
    • ACLY_HUMAN antibody
    • ATP citrate (pro-S) lyase antibody
    • ATP citrate lyase antibody
    • ATP citrate synthase antibody
    • ATP-citrate (pro-S-)-lyase antibody
    • ATP-citrate synthase antibody
    • ATPcitrate synthase antibody
    • ATPCL antibody
    • Citrate cleavage enzyme antibody
    • CLATP antibody
    • OTTHUMP00000164773 antibody
    see all

Images

  • All lanes : Anti-ATP citrate lyase antibody (ab157098) at 0.4 µg/ml

    Lane 1 : Jurkat whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 120 kDa


    Exposure time: 30 seconds
  • All lanes : Anti-ATP citrate lyase antibody (ab157098) at 0.4 µg/ml

    Lane 1 : NIH3T3 whole cell lysate at 50 µg
    Lane 2 : NIH3T3 whole cell lysate at 15 µg

    Developed using the ECL technique.

    Predicted band size: 120 kDa


    Exposure time: 10 seconds

References

This product has been referenced in:
  • Huang C  et al. Soy Isoflavones Regulate Lipid Metabolism through an AKT/mTORC1 Pathway in Diet-Induced Obesity (DIO) Male Rats. Molecules 21:N/A (2016). Read more (PubMed: 27153053) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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