Anti-ATP citrate lyase antibody (ab235926)
Key features and details
- Rabbit polyclonal to ATP citrate lyase
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ATP citrate lyase antibody
See all ATP citrate lyase primary antibodies -
Description
Rabbit polyclonal to ATP citrate lyase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human ATP citrate lyase aa 4-265.
Database link: P53396 -
Positive control
- IHC-P: Human breast cancer, gastric cancer and testis tissue. ICC/IF: HepG2 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/20 - 1/200.
|
|
ICC/IF |
1/50 - 1/200.
|
Notes |
---|
IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
-
Function
ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. -
Sequence similarities
In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
Contains 1 ATP-grasp domain. -
Post-translational
modificationsISGylated.
Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 47 Human
- Omim: 108728 Human
- SwissProt: P53396 Human
- Unigene: 387567 Human
-
Alternative names
- ACL antibody
- Acly antibody
- ACLY_HUMAN antibody
see all
Images
-
Paraffin-embedded human testis tissue stained for ATP citrate lyase with ab235926 at a 1/100 dilution in immunohistochemical analysis.
-
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for ATP citrate lyase using ab235926 at a dilution of 1/100 in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
-
Paraffin-embedded human gastric cancer tissue stained for ATP citrate lyase with ab235926 at a 1/100 dilution in immunohistochemical analysis.
-
Paraffin-embedded human breast cancer tissue stained for ATP citrate lyase with ab235926 at a 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab235926 has not yet been referenced specifically in any publications.