Overview

  • Product name

    Anti-ATP citrate lyase antibody - C-terminal
    See all ATP citrate lyase primary antibodies
  • Description

    Rabbit polyclonal to ATP citrate lyase - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Rhesus monkey, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human ATP citrate lyase aa 1051-1101 (C terminal). The exact sequence is proprietary. (NP_001087.2).
    Database link: P53396

  • Positive control

    • WB: Jurkat, HeLa, HEK-293T and NIH/3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab226200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 120 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
    • Sequence similarities

      In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
      In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
      Contains 1 ATP-grasp domain.
    • Post-translational
      modifications

      ISGylated.
      Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
      Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • ACL antibody
      • Acly antibody
      • ACLY_HUMAN antibody
      • ATP citrate (pro-S) lyase antibody
      • ATP citrate lyase antibody
      • ATP citrate synthase antibody
      • ATP-citrate (pro-S-)-lyase antibody
      • ATP-citrate synthase antibody
      • ATPcitrate synthase antibody
      • ATPCL antibody
      • Citrate cleavage enzyme antibody
      • CLATP antibody
      • OTTHUMP00000164773 antibody
      see all

    Images

    • All lanes : Anti-ATP citrate lyase antibody - C-terminal (ab226200) at 0.4 µg/ml

      Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
      Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg
      Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 120 kDa


      Exposure time: 30 seconds
    • All lanes : Anti-ATP citrate lyase antibody - C-terminal (ab226200) at 0.4 µg/ml

      Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
      Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 15 µg

      Developed using the ECL technique.

      Predicted band size: 120 kDa


      Exposure time: 10 seconds

    References

    ab226200 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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