Western blot abreview for Anti-ATP citrate lyase antibody [EP704Y]

Good
Abreviews
Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Head)
Loading amount
40 µg
Specification
Head
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.5% milk

Secondary antibody

Name
Non-Abcam antibody was used: Licoer anti Rab-green
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/12000

Detection

Detection method
Licor machine
Exposure
1000 hour(s), 40 minute(s) and 39 second(s)
Bands
Specific: 122 kDa

Additional data

Abcam response
We wish to thank the reviewer for providing this Abreview for Drosphila melanogaster

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Submitted Oct 02 2012

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