Western blot abreview for Anti-ATP citrate lyase antibody [EP704Y]

Good
Abreviews
Application
Western blot
Loading amount
80 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Sample
Marmoset (common) Tissue lysate - whole (brain)
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% non-fat milk in TBST
Dilution
1/850

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/12000

Detection

Detection method
Chemiluminescence
Bands
Specific: 125 kDa
Exposure
1 minute(s) and 43 second(s)

Additional data

Additional Notes
Abtrial Program: ABTRIAL-TBRP9

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Submitted Jun 05 2014

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