Product nameATP-gamma-S, Kinase substrate
- ATP gamma S
Substrate and inhibitor of ATP-dependent enzyme systems. Hydrolyzed very slowly by phosphatases and most ATPases. Once thiophosphorylated, proteins are resistant to protein phosphatases. P2 purinergic receptor agonist.
Kinase reaction reagent to be used with ab92570 rabbit monoclonal to thiophosphate ester for the identification of direct kinase substrates as mentioned in Allen JJ et al. Nat Methods 4:511-6 (2007).
After the kinase of interest has accepted ATP-γ-S, p-Nitrobenzyl mesylate (ab138910) can be used to alkylate the thiophospholyation site on the substrates. A thiophosphate ester rabbit monoclonal antibody (ab92570) is introduced to identify the tagged substrates.
It is suggested to add 100 ng of ATP-γ-S to a 30 µL kinase buffer. See Allen JJ et al. Nat Methods 4:511-6 (2007); Supp. Material.
Chemical nameAdenosine 5'-(3-thiotriphosphate) tetralithium salt
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 10 mM
This product is supplied in one (or more) pack size which is freeze dried. Therefore the contents may not be readily visible, as they can coat the bottom or walls of the vial. Please see our FAQs and information page for more details on handling.
Unstable; make up solutions fresh and use immediately.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab138911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab138911 has been referenced in 3 publications.
- Guo J et al. AKT methylation by SETDB1 promotes AKT kinase activity and oncogenic functions. Nat Cell Biol 21:226-237 (2019). PubMed: 30692625
- Lertsuwan J & Ruchirawat M Inhibitory Effects of ATP and Adenosine on Cholangiocarcinoma Cell Proliferation and Motility. Anticancer Res 37:3553-3561 (2017). PubMed: 28668846
- Allen JJ et al. A semisynthetic epitope for kinase substrates. Nat Methods 4:511-6 (2007). PubMed: 17486086