Overview

  • Product name

    Anti-ATP synthase C antibody [EPR13907]
    See all ATP synthase C primary antibodies
  • Description

    Rabbit monoclonal [EPR13907] to ATP synthase C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATP synthase C aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: P05496

  • Positive control

    • WB: Human fetal liver and fetal heart lysates; HL-60 cell lysate. ICC/IF: HL-60 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13907
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 8 kDa (predicted molecular weight: 14 kDa).
ICC/IF 1/100.

Target

  • Function

    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element.
  • Sequence similarities

    Belongs to the ATPase C chain family.
  • Cellular localization

    Mitochondrion membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AT5G1_HUMAN antibody
    • ATP synthase c subunit antibody
    • ATP synthase F(0) complex subunit C1, mitochondrial antibody
    • ATP synthase F(0) complex subunit C2, mitochondrial antibody
    • ATP synthase F(0) complex subunit C3, mitochondrial antibody
    • ATP synthase lipid binding protein mitochondrial antibody
    • ATP synthase lipid-binding protein antibody
    • ATP synthase proteolipid P1 antibody
    • ATP synthase proteolipid P2 antibody
    • ATP synthase proteolipid P3 antibody
    • ATP synthase proton-transporting mitochondrial F(0) complex subunit C1 antibody
    • ATP synthase proton-transporting mitochondrial F(0) complex subunit C2 antibody
    • ATP synthase proton-transporting mitochondrial F(0) complex subunit C3 antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9), isoform 1 antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9), isoform 2 antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c, isoform 1 antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c, isoform 2 antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c, isoform 3 antibody
    • ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C1 (subunit 9) antibody
    • ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C2 (subunit 9) antibody
    • ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C3 (subunit 9) antibody
    • ATP synthase, mitochondrial, C subunit-3 antibody
    • ATP5A antibody
    • ATP5G antibody
    • ATP5G1 antibody
    • ATPase protein 9 antibody
    • ATPase subunit 9 antibody
    • ATPase subunit c antibody
    • mitochondrial antibody
    • mitochondrial ATP synthase, subunit 9, isoform 1 antibody
    • mitochondrial ATP synthase, subunit 9, isoform 2 antibody
    • mitochondrial ATP synthase, subunit 9, isoform 3 antibody
    • mitochondrial ATP synthase, subunit C (subunit 9), isoform 2 antibody
    • mitochondrial ATP synthase, subunit C, isoform 1 antibody
    • mitochondrial ATP synthase, subunit C, isoform 2 antibody
    • mitochondrial ATP synthase, subunit C, isoform 3 antibody
    • P3 antibody
    see all

Images

  • All lanes : Anti-ATP synthase C antibody [EPR13907] (ab181243) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : HL-60 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 14 kDa
    Observed band size: 8 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescent analysis of acetone-fixed HL-60 cells labeling ATP synthase C with ab181243 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.

References

This product has been referenced in:

  • Poppens MJ  et al. Tracking sex-dependent differences in a mouse model of CLN6-Batten disease. Orphanet J Rare Dis 14:19 (2019). Read more (PubMed: 30665444) »
  • Sima N  et al. Neural stem cells for disease modeling and evaluation of therapeutics for infantile (CLN1/PPT1) and late infantile (CLN2/TPP1) neuronal ceroid lipofuscinoses. Orphanet J Rare Dis 13:54 (2018). ICC/IF . Read more (PubMed: 29631617) »
See all 4 Publications for this product

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