Recombinant
RabMAb

Recombinant Anti-ATP synthase C antibody [EPR13907] (Alexa Fluor® 555) (ab210732)

Overview

  • Product name

    Anti-ATP synthase C antibody [EPR13907] (Alexa Fluor® 555)
    See all ATP synthase C primary antibodies
  • Description

    Rabbit monoclonal [EPR13907] to ATP synthase C (Alexa Fluor® 555)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATP synthase C aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: P05496

  • Positive control

    • ICC/IF: HepG2 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13907
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab210732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.

This product gave a positive signal in HepG2 cells fixed with 100% methanol (5 min)

Target

  • Function

    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element.
  • Sequence similarities

    Belongs to the ATPase C chain family.
  • Cellular localization

    Mitochondrion membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AT5G1_HUMAN antibody
    • ATP synthase c subunit antibody
    • ATP synthase F(0) complex subunit C1, mitochondrial antibody
    • ATP synthase F(0) complex subunit C2, mitochondrial antibody
    • ATP synthase F(0) complex subunit C3, mitochondrial antibody
    • ATP synthase lipid binding protein mitochondrial antibody
    • ATP synthase lipid-binding protein antibody
    • ATP synthase proteolipid P1 antibody
    • ATP synthase proteolipid P2 antibody
    • ATP synthase proteolipid P3 antibody
    • ATP synthase proton-transporting mitochondrial F(0) complex subunit C1 antibody
    • ATP synthase proton-transporting mitochondrial F(0) complex subunit C2 antibody
    • ATP synthase proton-transporting mitochondrial F(0) complex subunit C3 antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9), isoform 1 antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9), isoform 2 antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c, isoform 1 antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c, isoform 2 antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c, isoform 3 antibody
    • ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C1 (subunit 9) antibody
    • ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C2 (subunit 9) antibody
    • ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C3 (subunit 9) antibody
    • ATP synthase, mitochondrial, C subunit-3 antibody
    • ATP5A antibody
    • ATP5G antibody
    • ATP5G1 antibody
    • ATPase protein 9 antibody
    • ATPase subunit 9 antibody
    • ATPase subunit c antibody
    • mitochondrial antibody
    • mitochondrial ATP synthase, subunit 9, isoform 1 antibody
    • mitochondrial ATP synthase, subunit 9, isoform 2 antibody
    • mitochondrial ATP synthase, subunit 9, isoform 3 antibody
    • mitochondrial ATP synthase, subunit C (subunit 9), isoform 2 antibody
    • mitochondrial ATP synthase, subunit C, isoform 1 antibody
    • mitochondrial ATP synthase, subunit C, isoform 2 antibody
    • mitochondrial ATP synthase, subunit C, isoform 3 antibody
    • P3 antibody
    see all

Images

  • ab210732 staining ATP synthase C in HepG2 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210732 at 1/50 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab210732 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab210732.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up