Recombinant Anti-ATP synthase C antibody [EPR13907] - BSA and Azide free (ab232644)
![Immunocytochemistry/ Immunofluorescence - Anti-ATP synthase C antibody [EPR13907] - BSA and Azide free (ab232644)](https://www.abcam.com/ps/products/232/ab232644/Images/ab232644-308989-anti-atp-synthase-c-antibody-epr13907-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ATP synthase C antibody [EPR13907] - BSA and Azide free (ab232644)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13907] to ATP synthase C - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ATP synthase C antibody [EPR13907] - BSA and Azide free
See all ATP synthase C primary antibodies -
Description
Rabbit monoclonal [EPR13907] to ATP synthase C - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ATP synthase C aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: P05496 -
Positive control
- ICC/IF: HL-60 cells.
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General notes
Ab232644 is the carrier-free version of ab181243. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab232644 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13907 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab232644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 8 kDa (predicted molecular weight: 14 kDa). | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element. -
Sequence similarities
Belongs to the ATPase C chain family. -
Cellular localization
Mitochondrion membrane. - Information by UniProt
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Database links
- Entrez Gene: 516 Human
- Entrez Gene: 517 Human
- Entrez Gene: 518 Human
- Entrez Gene: 100039108 Mouse
- Entrez Gene: 100043121 Mouse
- Entrez Gene: 100504871 Mouse
- Entrez Gene: 11951 Mouse
- Entrez Gene: 228033 Mouse
see all -
Alternative names
- AT5G1_HUMAN antibody
- ATP synthase c subunit antibody
- ATP synthase F(0) complex subunit C1, mitochondrial antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ATP synthase C antibody [EPR13907] - BSA and Azide free (ab232644)
Immunofluorescent analysis of acetone-fixed HL-60 (human promyelocytic leukemia cell line) cells labeling ATP synthase C with ab181243 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with DAPI.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181243).
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![Anti-ATP synthase C antibody [EPR13907] - BSA and Azide free (ab232644)](https://www.abcam.com/ps/products/232/ab232644/Images/ab232644-2-benefits-of-recombinant-antibodies.png)
Anti-ATP synthase C antibody [EPR13907] - BSA and Azide free (ab232644)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab232644 has not yet been referenced specifically in any publications.
