For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
ab109714 ATP synthase Enzyme Activity Microplate Assay Kit is used to determine the activity of ATP synthase (Complex V) in a human or rat sample. The ATP synthase enzyme is immunocaptured within the wells of the microplate and the enzyme activity is measured by monitoring the decrease in absorbance at 340 nm. Specifically, the conversion of ATP to ADP by ATP synthase is coupled to the oxidation reaction of NADH to NAD+ with a reduction in absorbance at 340 nm.
Store Buffer (Tube 1), Detergent, and the covered microplate at 4°C. Store the Lipid Mix and Reagent Mix at -80°C. For multiple experiments the Reagent Mix may be aliquoted at -80°C.
|ATP synthase precoated Microtiter plate||1 unit|
|Buffer||Tube 1||1 x 10ml|
|Detergent||1 x 1ml|
|Phospholipids||1 x 6ml|
|Reagent Mix||1 x 20ml|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"