ATP synthase Enzyme Activity Microplate Assay Kit (ab109714)


  • Product name
    ATP synthase Enzyme Activity Microplate Assay Kit
    See all ATP Synthase kits
  • Detection method
  • Sample type
    Cell culture extracts, Tissue, Suspension cells, Tissue Extracts, Cell Lysate, Purified mitochondria
  • Assay type
    Enzyme activity
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat, Cow, Human
    Does not react with: Mouse
  • Product overview

    ab109714 ATP synthase Enzyme Activity Microplate Assay Kit is used to determine the activity of ATP synthase (Complex V) in a human or rat sample. The ATP synthase enzyme is immunocaptured within the wells of the microplate and the enzyme activity is measured by monitoring the decrease in absorbance at 340 nm. Specifically, the conversion of ATP to ADP by ATP synthase is coupled to the oxidation reaction of NADH to NAD+ with a reduction in absorbance at 340 nm.


  • Notes

    Store Buffer (Tube 1), Detergent, and the covered microplate at 4°C. Store the Lipid Mix and Reagent Mix at -80°C. For multiple experiments the Reagent Mix may be aliquoted at -80°C.

  • Platform



  • Examples of activity/load relationships for cultured cell lysate, rat liver mitochondria, and bovine heart mitochondria.
  • Functional study using ATP synthase Enzyme Activity Microplate Assay Kit (ab109714).
  • Principle of ATP synthase Enzyme Activity Microplate Assay Kit (ab109714).



This product has been referenced in:
  • Merz TM  et al. Mitochondrial function of immune cells in septic shock: A prospective observational cohort study. PLoS One 12:e0178946 (2017). Functional Studies . Read more (PubMed: 28591158) »
  • Sun S  et al. Loss of the novel mitochondrial protein FAM210B promotes metastasis via PDK4-dependent metabolic reprogramming. Cell Death Dis 8:e2870 (2017). Read more (PubMed: 28594398) »

See all 12 Publications for this product

Customer reviews and Q&As

Clear instructions, easy to standardize, good specificity with microplane readers, reproducible results.

Abcam user community

Verified customer

Submitted Feb 27 2018

Thank you for your telephone enquiry earlier today.

I contacted the laboratory who confirmed that the 10X detergent included with this kit is 5% Lauryl Maltoside. The closest product we have is 10% Lauryl Maltoside Solution (ab109857). https:...

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Yes, typically researchers have used citrate synthase activity to normalize the quality of sample preparation. However, it is also well known in the literature that cell lines with defects in the OXPHOS complexes have increase citrate synthase activity...

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- No hay ningún problema en realizar el ensayo a 37 ºC. Simplemente irá mas rápido y no podréis comparar los resultados obtenidos con los de la datasheet, o con publicaciones anteriores que pudieran resultaros...

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En respuesta a las preguntas formuladas acerca del kit ab109714:

- Una de ellas hace referencia a la temperatura, ¿por qué el ensayo es a 30ºC? ¿Las enzimas que se utilizan no son de mamífero? El ens...

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Any color development is not visible to the naked eye but should easily be detectable to the plate reader.

The wavelength looks right 340nm and there should be a sizeable absorbance due to NADH abs at 340nm (we would expect 0.5), lo...

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Thank you for contacting us.Please find the replies to your questions below.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

1. H...

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Product code: 109714 Inquiry: Hi Abcam, Do you know if this kit will immunocapture the ATP synthase enzyme from C. elegans? Unlikely unfortunately – this kit was tested positive with human, rat and cow. Mouse was negative. I think it is unl...

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Thank you for your response.

You can certainly freeze down the samples if you wish. I have attached a new version of the Protocol which will be updated/downloaded on the on-line product datasheet as well.

If you need any further ass...

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Thank you for your enquiry and your interest in our products.

I would suggest trypsinizing the cells to detach them from the culture dish/well or using a cell scraper.

Afterwards the cells can be pelleted and washed, then brought up...

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