Anti-ATP synthase Immunocapture antibody [12F4AD8AF8] (ab109867)


  • Product name
    Anti-ATP synthase Immunocapture antibody [12F4AD8AF8]
  • Description
    Mouse monoclonal [12F4AD8AF8] to ATP synthase Immunocapture
  • Host species
  • Tested applications
    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Bovine ATP synthase

  • Positive control
  • General notes

    This antibody clone is manufactured by Abcam.


    ab109867 is a sample of pure immunocapture antibody, not immobilized to a solid support.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact or you can find further information here.



Our Abpromise guarantee covers the use of ab109867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. PubMed: 22523357
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


IP Use at an assay dependent concentration. 100 µg ab109867 can capture at least 50 µg ATP synthase Immunocapture (determined with 1 mg solubilized bovine heart mitochondria).


  • Relevance
    Complex V, also called F1F0ATPase or ATP synthase, is responsible for ATP production in oxidative phosphorylation and can work in reverse as a proton pumping ATPase. The enzyme was thought to be localized exclusively to mitochondria. However, it has recently been identified on the plasma membrane of several cell types including hepatocytes where it functions as the HDL receptor, on endothelial cells where it may act as the angiostatin receptor, and on the surface of cancer cells. The enzyme in mammals is composed of 17 subunits, five of which make up the easily detached F1. The remainder subunits are components of two stalk domains and the proton pumping F0 part of the machinery. Two of the subunits of the F0 part are encoded on mitochondrial DNA while the other subunits are nuclear encoded. Mutations in the mitochondrial-encoded subunits of ATP synthase (Complex V) cause OXPHOS disease.


  • Using the immunoprecipitation protocol provided, ATP synthase was isolated from various samples by antibody ab109867 crosslinked to protein G-agarose beads (ab109715). Protein bands identities were confirmed by mass spectrometry.
  • Overlay histogram showing HepG2 cells stained with ab109867 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109867, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


This product has been referenced in:

See all 12 Publications for this product

Customer reviews and Q&As

Immuno-precipitation step
Protein A/G
Mouse Tissue lysate - whole (Brain mitochondria)
Brain mitochondria
Total protein in input
300 µg

Abcam user community

Verified customer

Submitted Jul 18 2014

The antibody was raised against the cow purified whole enzyme to maximize affinity for native active holoenzyme.

1) Our best immunocapture/immunoprecipitation antibodies bind the native folded proteins so often do not work in Western blot...

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Thank you for your reply.

I'm glad you found the information provided useful. You can use whole cell lysates with the antibody.However, it is critical to use LM as the detergent though. Other detergents such as NP40 or SDS in RIPA bufferwill ...

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Thank you for contacting us and sorry for the delay in getting back to you.

I have contacted Mitosciences and they have shared the following protocols which can be used with the Anti-ATP synthase Immunocapture antibody [12F4AD8AF8] (ab109867)...

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Abcam has not validated the combination of species/application used in this Abreview.
IHC - Wholemount
Fruit fly (Drosophila melanogaster) Tissue (Dissected 3rd instar larvae)
Dissected 3rd instar larvae

Abcam user community

Verified customer

Submitted May 01 2012

Thank you for your call today and for your interest in ab109867. Please see below for information about your testing discount code, and please let me know if you have any questions.

Expiration date: July 21, 2012

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Thank you for contacting us. The immunogen was not a recombinant protein.  It was purified ATP synthase enzyme from bovine heart (the whole complex).  Hence we don’t know the exact epitope to which the protein binds.  However, we do know for certain it...

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