Product nameATP Synthase Immunocapture Kit
See all ATP Synthase kits
Species reactivityReacts with: Mouse, Rat, Cow, Human
250 µg, 500 µg or 750 µg of monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture up to ~125 µg, ~250 µg or ~500 µg respectively of ATP synthase (Complex V) from heart mitochondria.
ab109715 allows isolation of the ATP synthase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains olygomycin sensitive ATP hydrolysis activities after isolation. Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Potential uses for ab109715 include but are not limited to examining alterations of ATP synthase subunits in inherited mitochondrial diseases, Alzheimer's disease, angiogenesis, hypertension and aging.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
Storage instructionsStore at +4°C. Please refer to protocols.
Components 250 µg 500 µg 750 µg ATP synthase monoclonal antibody coupled agarose beads 1 x 250µg 2 x 250µg 1 x 750µg
- Complex V
- F1F0 ATP synthase
Using the immunoprecipitation protocol provided, ATP synthase was isolated from various samples by antibody ab109867 crosslinked to protein G-agarose beads (ab109715). Protein bands identities were confirmed by mass spectrometry.
This product has been referenced in:
- Ludtmann MHR et al. a-synuclein oligomers interact with ATP synthase and open the permeability transition pore in Parkinson's disease. Nat Commun 9:2293 (2018). Read more (PubMed: 29895861) »
- Sheeran FL & Pepe S Posttranslational modifications and dysfunction of mitochondrial enzymes in human heart failure. Am J Physiol Endocrinol Metab 311:E449-60 (2016). Read more (PubMed: 27406740) »