Anti-ATP11A antibody (ab105351)
Key features and details
- Rabbit polyclonal to ATP11A
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATP11A antibody -
Description
Rabbit polyclonal to ATP11A -
Host species
Rabbit -
Specificity
ab105351 is predicted to not cross react with AT11B. At least two isoforms of ATP11A are known to exist; this antibody will recognize both. -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ATP11A (N terminal).
Database link: NP_115565 -
Positive control
- WB: K562 cell lysate ICC: K562 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 130 kDa.
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ICC |
Use a concentration of 10 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 130 kDa. |
ICC
Use a concentration of 10 - 20 µg/ml. |
Target
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Function
Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules (Probable). May be involved in the uptake of farnesyltransferase inhibitor drugs, such as lonafarnib. -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. -
Cellular localization
Cell membrane. Early endosome. Recycling endosome. Endoplasmic reticulum. Exit from the endoplasmic reticulum requires the presence of TMEM30A, but not TMEM30B. In the presence of TMEM30A, predominantly located in the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 23250 Human
- Omim: 605868 Human
- SwissProt: P98196 Human
- Unigene: 29189 Human
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Form
P-type ATPases, such as ATP11A, are phosphorylated in their intermediate state and drive uphill transport of ions across membranes. -
Alternative names
- AT11A antibody
- AT11A_HUMAN antibody
- Atp11a antibody
see all
Images
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Immunofluorescence of ATP11A in K562 cells with ab105351 at 20 ug/mL.
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All lanes : Anti-ATP11A antibody (ab105351) at 1 µg/ml
Lane 1 : K562 cell lysate
Lane 2 : K562 cell lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 130 kDa -
ab105351, at 10 µg/ml, staining ATP11A in K562 cells by Immunocytochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab105351 has not yet been referenced specifically in any publications.