Overview

  • Product name

  • Description

    Rabbit polyclonal to ATP11B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    19 amino acid synthetic peptide near the N-terminus of Human ATP11B (NP_055431).

  • Positive control

    • K562 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab105377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 134 kDa.
ICC Use a concentration of 10 µg/ml.

Target

Images

  • Immunofluorescence of ATP11B in K562 cells with ab105377 at 20 ug/mL.

  • All lanes : Anti-ATP11B antibody (ab105377) at 1 µg/ml

    Lane 1 : K562 cell tissue lysate without blocking peptide
    Lane 2 : K562 cell tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 134 kDa

  • ab105377 at 10 µg/ml staining ATP11B in K562 cells by Immunocytochemistry

References

ab105377 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your reply and thank you for trying the mouse samples as well! I am very sorry that these3 antibodies did not perform as expected. I would be happy to offer you either a free of charge replacement for each antibody, or a credit or refund. For AT11A, we only have on other antibody in the catalog (ab117577) which has not yet been tested in mouse. For AT11B, we 2 other antibodies that are predicted to work in mouse, but also have not been tested with this species: ab82640 and ab117580. For AT11C, we have no other antibody available. I can offer you a credit or refund in this case or a replacement with an antibody to a different protein. In case of AT11A and AT11B, we are not able to guarantee the replacement antibodies for mouse (as they have not been tested), but I can give you a testing discount code for each replacement antibody. Alternatively, a different lot can be tried for ab105377 (AT11B). For the other 2 antibodies we currently have no vial in stock and I am not sure if the same or a new lot would come in next. Some more information on our testing discount program, should you decide to chose the antibodies that are not yet tested in mouse: For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. Please let me know how you would like to proceed and I'd be happy to arrange it for you. I look forward to hear back from you and resolve this case for you.

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Answer

Thank you for your phone call. I am sorry to hear that these antibodies are not providing satisfactory results so far. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Having reviewed this case, I would like to offer some suggestions to help optimize the results, as we discussed over the phone: 1) Since bovine samples have not been tested we cannot guarantee the antibodies for this species. However, mouse samples have been tested and you said you have mouse samples available to you. Thus, trying the antibodies on the mouse samples would be advisable to do. 2) Since high background was observed, using 5% milk for 2h (instead of 1% milk for 30 min) could help improve the signal and reduce the background. 3) Also, diluting the primary antibody in milk blocking solution can help as well. Should these suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.

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