Overview

  • Product name

  • Description

    Rabbit polyclonal to ATP11C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 667-716 (GLKVWVLTGD KMETAKSTCY ACRLFQTNTE LLELTTKTIE ESERKEDRLH) of Mouse ATP11C (NM_001037863).

  • Positive control

    • Mouse brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab110710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 129 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AT11C_HUMAN antibody
    • ATP11C antibody
    • ATPase class VI type 11C antibody
    • ATPase IG antibody
    • ATPase IQ antibody
    • ATPIG antibody
    • ATPIQ antibody
    • phospholipid-transporting ATPase IG antibody
    • Probable phospholipid-transporting ATPase IG antibody
    • RP11-197K18.1 antibody
    see all

Images

  • Anti-ATP11C antibody (ab110710) at 1 µg/ml + Mouse brain lysate at 10 µg

    Predicted band size: 129 kDa



    Gel concentration: 6-18%.

References

ab110710 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question

This email has 2 sections: A recap of the complaint The respondent’s survey results (section-by-section) Recap of the complaint --------------------------------------------------------------------------------------------------------------------- Date(s) submitted: 03-Nov-11 Catalog number(s): 110710 Product(s): Rabbit polyclonal to ATP11C CCEID: 3262659 Survey results --------------------------------------------------------------------------------------------------------------------- Telephone section Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint? A. Yes Q. Was it clear from the phone options which number you needed to press to get your issue resolved? A. Yes Protocol advice section Q. Did Abcam's Scientific Support give you protocol advice? A.Yes Q. How did you feel about being given protocol advice? A.I requested protocol advice Q. Did the product work successfully after following the advice? A.No Q. Why have you not tried the protocol advice? (Select all that apply.) A. A. A. A. Free of charge replacement section Q. Did Abcam's Scientific Support give you a free of charge replacement? A. Yes Q. Did the replacement product work successfully? A.No Credit note / refund section Q. Did Abcam's Scientific Support give you a credit note or a refund? A.Yes Q. Would you have preferred a different outcome from receiving a credit note or refund? A.No Overall satisfaction section Q. How satisfied are you with how the complaint was handled? A.Satisfied Q. Could we have done anything to better resolve the problem? A. Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution? A.No, I would not mind Q. Please provide a phone number or email address where we can reach you. A. Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products. A.

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Answer

Thank you for participating in our recent surevy. If there is anything else I can do for you, please just let me know. I'd be happy to assist you.

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Answer

Thank you for your reply and thank you for trying the mouse samples as well! I am very sorry that these3 antibodies did not perform as expected. I would be happy to offer you either a free of charge replacement for each antibody, or a credit or refund. For AT11A, we only have on other antibody in the catalog (ab117577) which has not yet been tested in mouse. For AT11B, we 2 other antibodies that are predicted to work in mouse, but also have not been tested with this species: ab82640 and ab117580. For AT11C, we have no other antibody available. I can offer you a credit or refund in this case or a replacement with an antibody to a different protein. In case of AT11A and AT11B, we are not able to guarantee the replacement antibodies for mouse (as they have not been tested), but I can give you a testing discount code for each replacement antibody. Alternatively, a different lot can be tried for ab105377 (AT11B). For the other 2 antibodies we currently have no vial in stock and I am not sure if the same or a new lot would come in next. Some more information on our testing discount program, should you decide to chose the antibodies that are not yet tested in mouse: For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. Please let me know how you would like to proceed and I'd be happy to arrange it for you. I look forward to hear back from you and resolve this case for you.

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Answer

Thank you for your phone call. I am sorry to hear that these antibodies are not providing satisfactory results so far. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Having reviewed this case, I would like to offer some suggestions to help optimize the results, as we discussed over the phone: 1) Since bovine samples have not been tested we cannot guarantee the antibodies for this species. However, mouse samples have been tested and you said you have mouse samples available to you. Thus, trying the antibodies on the mouse samples would be advisable to do. 2) Since high background was observed, using 5% milk for 2h (instead of 1% milk for 30 min) could help improve the signal and reduce the background. 3) Also, diluting the primary antibody in milk blocking solution can help as well. Should these suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.

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