Anti-ATP13A1 antibody (ab230786)
Key features and details
- Goat polyclonal to ATP13A1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATP13A1 antibody -
Description
Goat polyclonal to ATP13A1 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Monkey -
Immunogen
Synthetic peptide within Human ATP13A1 aa 950-1050 (internal sequence) (Cysteine residue). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_065143.2).
Database link: Q9HD20 -
Positive control
- IHC-P: Human thyroid tissue. WB: Human kidney lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 133 kDa.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 133 kDa. |
Target
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Relevance
ATP13A1 belongs to the cation transport ATPase (P type) family. Type V subfamily. There are three named isoforms produced by alternative splicing. -
Cellular localization
Membrane; Multi pass membrane protein. -
Database links
- Entrez Gene: 534369 Cow
- Entrez Gene: 57130 Human
- Entrez Gene: 170759 Mouse
- Entrez Gene: 290673 Rat
- SwissProt: Q9HD20 Human
- SwissProt: Q9EPE9 Mouse
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Alternative names
- ATP13A antibody
- ATPase type 13A antibody
- ATPase type 13A1 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human thyroid tissue stained for ATP13A1 using ab230786 at 5 µg/ml in immunohistochemical analysis.
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Anti-ATP13A1 antibody (ab230786) at 0.3 µg/ml + Human kidney lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 133 kDaPrimary incubation was 1 hour.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab230786 has not yet been referenced specifically in any publications.