Recombinant
RabMAb

Recombinant Anti-ATP1A3 antibody [EPR14137] - N-terminal (ab182572)

Overview

  • Product name
    Anti-ATP1A3 antibody [EPR14137] - N-terminal
    See all ATP1A3 primary antibodies
  • Description
    Rabbit monoclonal [EPR14137] to ATP1A3 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ATP1A3 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P13637
    (Peptide available as ab205970)

  • Positive control
    • Mouse and rat brain lysate. Human fetal brain lysate. Human hippocampus tissue lysate.
  • General notes

     This product was previously labelled as alpha 3 Sodium Potassium ATPase

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab182572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 100-150 kDa (predicted molecular weight: 111 kDa).

Target

  • Function
    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • Involvement in disease
    Dystonia 12
    Alternating hemiplegia of childhood 2
    Cerebellar ataxia, areflexia, pes cavus, optic atrophy, and sensorineural hearing loss
  • Sequence similarities
    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHC2 antibody
    • Alpha(III) antibody
    • AT1A3_HUMAN antibody
    • Atp1a3 antibody
    • ATPase Na+/K+ transporting alpha 3 polypeptide antibody
    • DYT 12 antibody
    • DYT12 antibody
    • MGC13276 antibody
    • Na(+)/K(+) ATPase alpha(III) subunit antibody
    • Na(+)/K(+) ATPase alpha-3 subunit antibody
    • Na+/K+ ATPase 3 antibody
    • Na+/K+ ATPase alpha 3 subunit antibody
    • RDP antibody
    • Sodium potassium ATPase alpha 3 polypeptide antibody
    • Sodium pump 3 antibody
    • Sodium pump subunit alpha-3 antibody
    • Sodium/potassium transporting ATPase alpha 3 chain antibody
    • Sodium/potassium-transporting ATPase subunit alpha-3 antibody
    see all

Images

  • All lanes : Anti-ATP1A3 antibody [EPR14137] - N-terminal (ab182572) at 1/5000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Human fetal brain lysate
    Lane 4 : Human hippocampus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 111 kDa

References

ab182572 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formalin 10%

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Verified customer

Submitted May 23 2018

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