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  1. Link

    atp1a3-antibody-epr14138-ab182571.pdf

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Neuroscience Neurotransmission Receptors / Channels Potassium Channels
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RecombinantRabMAb

Recombinant Anti-ATP1A3 antibody [EPR14138] (ab182571)

  • Datasheet
  • Certificate of Compliance
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Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [EPR14138] (ab182571)
  • Immunoprecipitation - Anti-ATP1A3 antibody [EPR14138] (ab182571)
  • Western blot - Anti-ATP1A3 antibody [EPR14138] (ab182571)
  • Anti-ATP1A3 antibody [EPR14138] (ab182571)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14138] to ATP1A3
  • Suitable for: IP, WB, ICC/IF
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

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Secondary
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View more associated products

Overview

  • Product name

    Anti-ATP1A3 antibody [EPR14138]
    See all ATP1A3 primary antibodies
  • Description

    Rabbit monoclonal [EPR14138] to ATP1A3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and Rat brain lysate. ICC/IF: Mouse primary neuron cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14138
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Potassium Channels
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Sodium Channels
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free (ab250624)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab4022)
    • Rat brain cerebellum tissue lysate - total protein (ab4032)
  • Recombinant Protein

    • Recombinant Human ATP1A3 protein (ab152203)
  • Related Products

    • Recombinant Human ATP1A3 protein (ab152203)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/20 - 1/40.
WB
1/1000. Detects a band of approximately 100-150 kDa (predicted molecular weight: 111 kDa).
ICC/IF
1/50.
Notes
IP
1/20 - 1/40.
WB
1/1000. Detects a band of approximately 100-150 kDa (predicted molecular weight: 111 kDa).
ICC/IF
1/50.

Target

  • Function

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • Involvement in disease

    Dystonia 12
    Alternating hemiplegia of childhood 2
    Cerebellar ataxia, areflexia, pes cavus, optic atrophy, and sensorineural hearing loss
  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P13637 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 478 Human
    • Entrez Gene: 232975 Mouse
    • Entrez Gene: 24213 Rat
    • Omim: 182350 Human
    • SwissProt: P13637 Human
    • SwissProt: Q6PIC6 Mouse
    • SwissProt: P06687 Rat
    • Unigene: 515427 Human
    • Unigene: 44101 Mouse
    • Unigene: 87329 Rat
    see all
  • Alternative names

    • AHC2 antibody
    • Alpha(III) antibody
    • AT1A3_HUMAN antibody
    • Atp1a3 antibody
    • ATPase Na+/K+ transporting alpha 3 polypeptide antibody
    • DYT 12 antibody
    • DYT12 antibody
    • MGC13276 antibody
    • Na(+)/K(+) ATPase alpha(III) subunit antibody
    • Na(+)/K(+) ATPase alpha-3 subunit antibody
    • Na+/K+ ATPase 3 antibody
    • Na+/K+ ATPase alpha 3 subunit antibody
    • RDP antibody
    • Sodium potassium ATPase alpha 3 polypeptide antibody
    • Sodium pump 3 antibody
    • Sodium pump subunit alpha-3 antibody
    • Sodium/potassium transporting ATPase alpha 3 chain antibody
    • Sodium/potassium-transporting ATPase subunit alpha-3 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [EPR14138] (ab182571)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [EPR14138] (ab182571)

    Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling ATP1A3 with purified ab182571 at 1/50 (5.2 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.

  • Immunoprecipitation - Anti-ATP1A3 antibody [EPR14138] (ab182571)
    Immunoprecipitation - Anti-ATP1A3 antibody [EPR14138] (ab182571)

    Western blot analysis of ATP1A3 in immunoprecipitation pellets from Mouse brain lysate. ab182571 used at 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.

  • Western blot - Anti-ATP1A3 antibody [EPR14138] (ab182571)
    Western blot - Anti-ATP1A3 antibody [EPR14138] (ab182571)
    All lanes : Anti-ATP1A3 antibody [EPR14138] (ab182571) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 111 kDa

  • Anti-ATP1A3 antibody [EPR14138] (ab182571)
    Anti-ATP1A3 antibody [EPR14138] (ab182571)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (1)

Publishing research using ab182571? Please let us know so that we can cite the reference in this datasheet.

ab182571 has been referenced in 1 publication.

  • Fernández ÁF  et al. Interaction between the autophagy protein Beclin 1 and Na+,K+-ATPase during starvation, exercise, and ischemia. JCI Insight 5:N/A (2020). PubMed: 31941841

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