Name: Anti-ATP1A3 antibody [EPR14138]
Brand: RabMAb
SKU: ab182571

Product name

Anti-ATP1A3 antibody [EPR14138]
See all ATP1A3 primary antibodies
Description

Rabbit monoclonal [EPR14138] to ATP1A3
Host species

Rabbit
Tested applications

Suitable for: IP, WBmore details
Species reactivity

Reacts with: Mouse, Rat
Predicted to work with: Human
Immunogen

Synthetic peptide within Human ATP1A3 aa 850-950 (internal sequence). The exact sequence is proprietary.
Database link: P13637
Positive control
- Mouse and Rat brain lysate.
General notes

This product was previously labelled as alpha 3 Sodium Potassium ATPase

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14138
Isotype

IgG
Research areas

Alternative Versions
- Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free (ab250624)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Mouse brain tissue lysate - total protein (ab4022)
- Rat brain cerebellum tissue lysate - total protein (ab4032)
Recombinant Protein
- Recombinant Human ATP1A3 protein (ab152203)
Related Products
- Recombinant Human ATP1A3 protein (ab152203)

Our Abpromise guarantee covers the use of ab182571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IP		1/20 - 1/40.
WB		1/1000. Detects a band of approximately 100-150 kDa (predicted molecular weight: 111 kDa).

Function

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Involvement in disease

Dystonia 12
Alternating hemiplegia of childhood 2
Cerebellar ataxia, areflexia, pes cavus, optic atrophy, and sensorineural hearing loss
Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
Cellular localization

Cell membrane.
Target information above from: UniProt accession P13637 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 478 Human
- Entrez Gene: 232975 Mouse
- Entrez Gene: 24213 Rat
- Omim: 182350 Human
- SwissProt: P13637 Human
- SwissProt: Q6PIC6 Mouse
- SwissProt: P06687 Rat
- Unigene: 515427 Human
- Unigene: 44101 Mouse
- Unigene: 87329 Rat
see all
Alternative names
- AHC2 antibody
- Alpha(III) antibody
- AT1A3_HUMAN antibody
- Atp1a3 antibody
- ATPase Na+/K+ transporting alpha 3 polypeptide antibody
- DYT 12 antibody
- DYT12 antibody
- MGC13276 antibody
- Na(+)/K(+) ATPase alpha(III) subunit antibody
- Na(+)/K(+) ATPase alpha-3 subunit antibody
- Na+/K+ ATPase 3 antibody
- Na+/K+ ATPase alpha 3 subunit antibody
- RDP antibody
- Sodium potassium ATPase alpha 3 polypeptide antibody
- Sodium pump 3 antibody
- Sodium pump subunit alpha-3 antibody
- Sodium/potassium transporting ATPase alpha 3 chain antibody
- Sodium/potassium-transporting ATPase subunit alpha-3 antibody
see all

Immunoprecipitation - Anti-ATP1A3 antibody [EPR14138] (ab182571)

Western blot analysis of ATP1A3 in immunoprecipitation pellets from Mouse brain lysate. ab182571 used at 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.
Western blot - Anti-ATP1A3 antibody [EPR14138] (ab182571)

All lanes : Anti-ATP1A3 antibody [EPR14138] (ab182571) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 111 kDa

Western blot protocols
Immunoprecipitation protocols

Datasheet

ab182571 has not yet been referenced specifically in any publications.

Publishing research using ab182571? Please let us know so that we can cite the reference in this datasheet.

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Recombinant Anti-ATP1A3 antibody [EPR14138] (ab182571)

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