Recombinant Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free (ab250624)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14138] to ATP1A3 - BSA and Azide free
- Suitable for: IP, WB, ICC/IF
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free
See all ATP1A3 primary antibodies -
Description
Rabbit monoclonal [EPR14138] to ATP1A3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Mouse primary neuron cells WB: rat and mouse brain lysates, unboiled.
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General notes
ab250624 is the carrier-free version of ab182571.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14138 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 100-150 kDa (predicted molecular weight: 111 kDa).
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 100-150 kDa (predicted molecular weight: 111 kDa). |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. -
Involvement in disease
Dystonia 12
Alternating hemiplegia of childhood 2
Cerebellar ataxia, areflexia, pes cavus, optic atrophy, and sensorineural hearing loss -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 478 Human
- Entrez Gene: 232975 Mouse
- Entrez Gene: 24213 Rat
- Omim: 182350 Human
- SwissProt: P13637 Human
- SwissProt: Q6PIC6 Mouse
- SwissProt: P06687 Rat
- Unigene: 515427 Human
see all -
Alternative names
- AHC2 antibody
- Alpha(III) antibody
- AT1A3_HUMAN antibody
see all
Images
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All lanes : Anti-ATP1A3 antibody [EPR14138] (ab182571) at 1/1000 dilution
Lane 1 : Mouse brain lysate, unboiled
Lane 2 : Mouse brain lysate, boiled
Lane 3 : Rat brain lysate, unboiled
Lane 4 : Rat brain lysate, boiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 111 kDa
Observed band size: 102 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182571).
Blocking and diluting buffer and concentration:5% NFDM/TBST.
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Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling ATP1A3 with purified ab182571 at 1/50 (5.2 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182571).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250624 has not yet been referenced specifically in any publications.