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  1. Link

    atp1a3-antibody-xvif9-g10-ab2826.pdf

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Neuroscience Neurotransmission Receptors / Channels Potassium Channels
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Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (13)

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Western blot - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Flow Cytometry - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

Key features and details

  • Mouse monoclonal [XVIF9-G10] to ATP1A3
  • Suitable for: WB, Flow Cyt, ICC, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

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Anti-Calnexin antibody (ab10286)
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Recombinant Human ATP1A3 protein (ab152203)
Secondary
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-ATP1A3 antibody [XVIF9-G10]
    See all ATP1A3 primary antibodies
  • Description

    Mouse monoclonal [XVIF9-G10] to ATP1A3
  • Host species

    Mouse
  • Specificity

    The immunogen used for this product shares 89% homology with ATP1A2. Cross-reactivity with this protein has not been confirmed experimentally
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Non human primates, Amphibian
  • Immunogen

    Full length native protein (purified) corresponding to Dog ATP1A3. Canine cardiac microsomes.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    XVIF9-G10
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Potassium Channels
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Sodium Channels
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human ATP1A3 protein (ab152203)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (4)
Use a concentration of 1 µg/ml. Predicted molecular weight: 111 kDa.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC (1)
Use a concentration of 5 µg/ml.
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 111 kDa.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC
Use a concentration of 5 µg/ml.
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • Involvement in disease

    Dystonia 12
    Alternating hemiplegia of childhood 2
    Cerebellar ataxia, areflexia, pes cavus, optic atrophy, and sensorineural hearing loss
  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P13637 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 478 Human
    • Entrez Gene: 232975 Mouse
    • Entrez Gene: 24213 Rat
    • Omim: 182350 Human
    • SwissProt: P13637 Human
    • SwissProt: Q6PIC6 Mouse
    • SwissProt: P06687 Rat
    • Unigene: 515427 Human
    • Unigene: 44101 Mouse
    • Unigene: 87329 Rat
    see all
  • Alternative names

    • AHC2 antibody
    • Alpha(III) antibody
    • AT1A3_HUMAN antibody
    • Atp1a3 antibody
    • ATPase Na+/K+ transporting alpha 3 polypeptide antibody
    • DYT 12 antibody
    • DYT12 antibody
    • MGC13276 antibody
    • Na(+)/K(+) ATPase alpha(III) subunit antibody
    • Na(+)/K(+) ATPase alpha-3 subunit antibody
    • Na+/K+ ATPase 3 antibody
    • Na+/K+ ATPase alpha 3 subunit antibody
    • RDP antibody
    • Sodium potassium ATPase alpha 3 polypeptide antibody
    • Sodium pump 3 antibody
    • Sodium pump subunit alpha-3 antibody
    • Sodium/potassium transporting ATPase alpha 3 chain antibody
    • Sodium/potassium-transporting ATPase subunit alpha-3 antibody
    see all

Images

  • Western blot - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Western blot - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    All lanes : Anti-ATP1A3 antibody [XVIF9-G10] (ab2826) at 1 µg/ml

    Lane 1 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 2 : IMR-32 (Human brain neuroblast cell line) whole cell lysate
    Lane 3 : SK-OV-3 (Human ovarian cancer cell line) whole cell lysate
    Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 5 : Mouse brain tissue lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

    Predicted band size: 111 kDa



    Samples were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel. Resolved proteins were then transferred onto a Nitrocellulose membrane by iBlot® 2 Dry Blotting System. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit.

  • Flow Cytometry - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Flow Cytometry - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Overlay histogram showing SH-SY5Y cells stained with ab2826 (red line). The cells were fixed with 4% paraformaldehyde and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2826, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human prostate carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at 1/50 dilution with ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at 1/200 dilution with ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at 1/200 dilution with ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

    Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using ab2826 shows staining in C6 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4 °C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

    Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using ab2826 shows staining in U251 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4 °C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

    Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using ab2826 shows staining in HeLa cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4°C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (13)

Publishing research using ab2826? Please let us know so that we can cite the reference in this datasheet.

ab2826 has been referenced in 13 publications.

  • Jiao S  et al. Comparative description of the mRNA expression profile of Na+ /K+ -ATPase isoforms in adult mouse nervous system. J Comp Neurol 530:627-647 (2022). PubMed: 34415061
  • Fernández ÁF  et al. Interaction between the autophagy protein Beclin 1 and Na+,K+-ATPase during starvation, exercise, and ischemia. JCI Insight 5:N/A (2020). PubMed: 31941841
  • Wang F  et al. Shikonin Inhibits Cancer Through P21 Upregulation and Apoptosis Induction. Front Pharmacol 11:861 (2020). PubMed: 32581812
  • Datta P  et al. The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors. J Biol Chem 294:19119-19136 (2019). PubMed: 31694913
  • Mota MVB  et al. ATP Synthase Subunit Beta Immunostaining is Reduced in the Sclerotic Hippocampus of Epilepsy Patients. Cell Mol Neurobiol 39:149-160 (2019). PubMed: 30539418
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a question

Question

Dear Abcam Team,

I am looking to perform some Immunohistochemistry experiments on rat myocardium and I was wondering whether you would recommend ab78798 or ab2826? We have not used any of these in our lab & I was looking for some guidance.

Thanks for your advice,
Yours,

Read More

Abcam community

Verified customer

Asked on Jan 17 2012

Answer

Thank you for your inquiry.

As our Abpromise guarantees the species and the application stated on the datasheet, I recommend to order ab78798 if you plan to use IHC-P and ab2826 if you plan to use IHC-Fr.

Both antibodies are tested in rat and therefore covered by our guarantee. Please follow this link to review the Abpromise guarantee:

https://www.abcam.com/index.html?pageconfig=abpromise

I would like to make you aware that matching secondary antibodies can be found using our search engine:

https://www.abcam.com/index.html?pageconfig=productmap&cl=918

And we also provide many IHC tailored products:

https://www.abcam.com/index.html?pageconfig=resource&rid=12853

I hope this information is helpful. Please do not hesitate to contact me again with more detailsabout the intendedexperiments if you have any further questions.

Read More

Abcam Scientific Support

Answered on Jan 17 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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