Western blot abreview for Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10]

Good
Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (Brain cortex)
Loading amount
10 µg
Specification
Brain cortex
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 24°C · Diluent: 5%milk in pbs-tw

Secondary antibody

Name
Non-Abcam antibody was used: santa cruz anti mouse IgG1
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 110 kDa

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Submitted Sep 09 2009

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