Western blot abreview for Anti-ATP1A3 antibody [XVIF9-G10]

Excellent
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (primary cortical neurons)
Gel Running Conditions
Reduced Denaturing
Treatment
0-35 days in culture
Specification
primary cortical neurons

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T 4% BSA
Dilution
1/1000

Secondary antibody

Dilution
1/20000
Name
Non-Abcam antibody was used: DAKO
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL femto kit from pierce
Exposure
5 second(s)
Bands
Specific: ~110 kDa Non-specific: ~70 kDa

Additional data

Additional Notes
very good signal, some faint background around 70-80 kD. For best results do not heat denature whole cell lysates as it may cause aggregation of the protein. Alternatively prepare plasma membrane fractions.

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Submitted Jan 23 2008

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