• Product name

    Anti-ATP1B1 antibody [464.8]
    See all ATP1B1 primary antibodies
  • Description

    Mouse monoclonal [464.8] to ATP1B1
  • Host species

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Rat, Cow, Dog, Human, Pig
  • Immunogen

    corresponding to ATP1B1.

  • Positive control

    • ab8344 gave a positive signal in human, mouse and rat kidney tissue lysates. In Flow Cytometry, this antibody gave a positive signal in methanol fixed/Tween permeabilised HepG2 cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

     This product was previously labelled as beta 1 Sodium Potassium ATPase




Our Abpromise guarantee covers the use of ab8344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


WB 1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 35 kDa).


  • Function

    This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
  • Tissue specificity

    Found in most tissues.
  • Sequence similarities

    Belongs to the X(+)/potassium ATPases subunit beta family.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Adenosinetriphosphatase antibody
    • AT1B1_HUMAN antibody
    • ATP 1B antibody
    • ATP1B antibody
    • ATP1B1 antibody
    • ATPase Na+/K+ transporting beta 1 polypeptide antibody
    • Atpb 1 antibody
    • ATPBS antibody
    • Beta 1 subunit of Na(+) K(+) ATPase antibody
    • Na K ATPase beta 1 polypeptide antibody
    • Na+/K+ ATPase beta 1 subunit antibody
    • Sodium/potassium dependent ATPase beta 1 subunit antibody
    • Sodium/potassium transporting ATPase beta 1 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 1 antibody
    • Sodium/potassium-dependent ATPase subunit beta-1 antibody
    • Sodium/potassium-transporting ATPase subunit beta-1 antibody
    see all


  • Anti-ATP1B1 antibody [464.8] (ab8344) at 1/500 dilution + Human kidney tissue lysate - total protein (ab30203) at 20 µg

    Granzyme B overexpression 293T lysate (whole cell) (ab94040) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 4 minutes
  • Overlay histogram showing HepG2 cells stained with ab8344 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8344, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


This product has been referenced in:

  • Wen X  et al. Gene-expression profile and localization of Na+/K(+)-ATPase in rat enamel organ cells. Eur J Oral Sci 122:21-6 (2014). Read more (PubMed: 24313748) »
  • Lu Q  et al. Ca²?/calmodulin-dependent protein kinase II contributes to hypoxic ischemic cell death in neonatal hippocampal slice cultures. PLoS One 8:e70750 (2013). WB ; Rat . Read more (PubMed: 23976956) »
See all 12 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Western blot
Rat Tissue lysate - whole (enamel organ)
Gel Running Conditions
Non-reduced Denaturing (4-12%)
Loading amount
15 µg
enamel organ
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Feb 18 2013


I can confirm that the laboratory has stopped using the Oddyssey blocking buffer as it gave a number of signal problems. We recommend to use 5% BSA or 5% milk, and both should work with the Oddyssey machine according to the lab. Can you please clarify whether the protocol of sucrose gradient is used with other proteins? I'm wondering if this step may be damaging the protein Sodium Potassium ATPase? If you still experience problems with the new blocking step please do not hesitate to contact me.

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Thank you for your enquiry. The epitope has not been mapped in the cases of ab7671 and ab8344 therefore we do not know which part of the protein is recognised by those antibodies. As the immunogen used to generate ab353 is purified Na,K-ATPase from rabbit renal outermedulla and the antibody is a polyclonal antibody the product will recognise a number of epitopes on the protein, some are likely to be at the plasma membrane portion intracellularly and some extracellularly. I hope this information helps, please do not hesitate to contact us again if you need more information,

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This antibody has been shown to cross react with Cow, Dog, Human, Pig and Rat. I'm afraid that, to our knowledge, it has not yet been tested in other species, so I do not know if it will work with CHO cells.

Read More

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