Anti-ATP1B1 antibody (ab96292)
Key features and details
- Rabbit polyclonal to ATP1B1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ATP1B1 antibody
See all ATP1B1 primary antibodies -
Description
Rabbit polyclonal to ATP1B1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Guinea pig, Dog, Pig -
Immunogen
Recombinant fragment corresponding to Human ATP1B1 aa 83-303.
Database link: P05026 -
Positive control
- MOLT4, A431, HepG2 and Raji cell lysates for WB and MDAMB468 xenograft for IHC-P
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
Optimal dilutions/concentrations should be determined by the researcher |
|
IHC-P |
1/100 - 1/1000.
Optimal dilutions/concentrations should be determined by the researcher |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa. Optimal dilutions/concentrations should be determined by the researcher |
IHC-P
1/100 - 1/1000. Optimal dilutions/concentrations should be determined by the researcher |
Target
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Function
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. -
Tissue specificity
Found in most tissues. -
Sequence similarities
Belongs to the X(+)/potassium ATPases subunit beta family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 403966 Dog
- Entrez Gene: 481 Human
- Entrez Gene: 396898 Pig
- Omim: 182330 Human
- SwissProt: P06583 Dog
- SwissProt: Q9JM72 Guinea pig
- SwissProt: P05026 Human
- SwissProt: P05027 Pig
see all -
Alternative names
- Adenosinetriphosphatase antibody
- AT1B1_HUMAN antibody
- ATP 1B antibody
see all
Images
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ab96292 detects ATP1B1 protein at membrane on human hepatoma by immunohistochemcial analysis. Sample: Paraffin-embedded hepatoma tissue. Antibody dilution: 1:500
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ab96292 detects ATP1B1 protein at membrane on Cal27 (human) xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. Antibody dilution: 1:500
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Anti-ATP1B1 antibody (ab96292) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 35 kDa
10% SDS-PAGE -
ab96292, at 1/100 dilution, staining beta Sodium Potassium ATPase in paraffin-embedded MDAMB468 (human) xenograft by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab96292 has been referenced in 1 publication.
- Su Y et al. Physical and Functional Links between Anion Exchanger-1 and Sodium Pump. J Am Soc Nephrol N/A:N/A (2014). Human . PubMed: 25012180