RecombinantRabMAb

Recombinant Anti-ATP1B1 antibody [EPR12195] (ab193669)

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Western blot - Anti-ATP1B1 antibody [EPR12195] (ab193669)
  • Western blot - Anti-ATP1B1 antibody [EPR12195] (ab193669)
  • Western blot - Anti-ATP1B1 antibody [EPR12195] (ab193669)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] (ab193669)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] (ab193669)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] (ab193669)
  • Anti-ATP1B1 antibody [EPR12195] (ab193669)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12195] to ATP1B1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ATP1B1 antibody [EPR12195]
    See all ATP1B1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12195] to ATP1B1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    within Human ATP1B1 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P05026
    (Peptide available as ab205969)

  • Positive control

    • Human fetal brain, fetal heart and fetal kidney lysates; Mouse brain and Rat heart lysates; Human renal adenocarcinoma tissue; Mouse liver tissue; Rat kidney tissue.

  • General notes

     This product was previously labelled as beta 1 Sodium Potassium ATPase

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab193669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45-50 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.

  • Tissue specificity

    Found in most tissues.

  • Sequence similarities

    Belongs to the X(+)/potassium ATPases subunit beta family.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Adenosinetriphosphatase antibody
    • AT1B1_HUMAN antibody
    • ATP 1B antibody
    • ATP1B antibody
    • ATP1B1 antibody
    • ATPase Na+/K+ transporting beta 1 polypeptide antibody
    • Atpb 1 antibody
    • ATPBS antibody
    • Beta 1 subunit of Na(+) K(+) ATPase antibody
    • Na K ATPase beta 1 polypeptide antibody
    • Na+/K+ ATPase beta 1 subunit antibody
    • Sodium/potassium dependent ATPase beta 1 subunit antibody
    • Sodium/potassium transporting ATPase beta 1 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 1 antibody
    • Sodium/potassium-dependent ATPase subunit beta-1 antibody
    • Sodium/potassium-transporting ATPase subunit beta-1 antibody
    see all

Images

  • Western blot - Anti-ATP1B1 antibody [EPR12195] (ab193669)
    Western blot - Anti-ATP1B1 antibody [EPR12195] (ab193669)
    Anti-ATP1B1 antibody [EPR12195] (ab193669) at 1/10000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 45-50 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ATP1B1 antibody [EPR12195] (ab193669)
    Western blot - Anti-ATP1B1 antibody [EPR12195] (ab193669)
    All lanes : Anti-ATP1B1 antibody [EPR12195] (ab193669) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 45-50 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ATP1B1 antibody [EPR12195] (ab193669)
    Western blot - Anti-ATP1B1 antibody [EPR12195] (ab193669)
    All lanes : Anti-ATP1B1 antibody [EPR12195] (ab193669) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 45-50 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] (ab193669)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] (ab193669)

    Immunohistochemical analysis of paraffin-embedded Human renal adenocarcinoma tissue labeling ATP1B1 with ab193669 at 1/100 dilution (7.8μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] (ab193669)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] (ab193669)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ATP1B1 with ab193669 at 1/100 dilution (7.8μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] (ab193669)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] (ab193669)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ATP1B1 with ab193669 at 1/100 dilution (7.8μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-ATP1B1 antibody [EPR12195] (ab193669)
    Anti-ATP1B1 antibody [EPR12195] (ab193669)

References (2)

Publishing research using ab193669? Please let us know so that we can cite the reference in this datasheet.

ab193669 has been referenced in 2 publications.

  • Jannas-Vela S  et al. Resting metabolic rate and skeletal muscle SERCA and Na+ /K+ ATPase activities are not affected by fish oil supplementation in healthy older adults. Physiol Rep 8:e14408 (2020). PubMed: 32342642
  • Han J  et al. Maresin Conjugates in Tissue Regeneration 1 improves alveolar fluid clearance by up-regulating alveolar ENaC, Na, K-ATPase in lipopolysaccharide-induced acute lung injury. J Cell Mol Med 24:4736-4747 (2020). PubMed: 32160403

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