Recombinant Anti-ATP1B1 antibody [EPR12195] (ab193669)

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Protein

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Primary - Rabbit Anti-ATP1B1 antibody [EPR12195] (ab193669)
WB, IHC-P

Protein - Recombinant human ATP1B1 protein (Active) (ab222961)
Functional Studies, SDS-PAGE

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Product name

Anti-ATP1B1 antibody [EPR12195]
See all ATP1B1 primary antibodies
Description

Rabbit monoclonal [EPR12195] to ATP1B1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

within Human ATP1B1 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: P05026
(Peptide available as ab205969)
Positive control
- Human fetal brain, fetal heart and fetal kidney lysates; Mouse brain and Rat heart lysates; Human renal adenocarcinoma tissue; Mouse liver tissue; Rat kidney tissue.
General notes

This product was previously labelled as beta 1 Sodium Potassium ATPase

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12195
Isotype

IgG
Research areas

Alternative Versions
- Anti-ATP1B1 antibody [EPR12195] - BSA and Azide free (ab251176)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Mouse brain tissue lysate - total protein (ab30151)
Recombinant Protein
- Recombinant human ATP1B1 protein (Active) (ab222961)

Our Abpromise guarantee covers the use of ab193669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Detects a band of approximately 45-50 kDa (predicted molecular weight: 35 kDa).
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Function

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Tissue specificity

Found in most tissues.
Sequence similarities

Belongs to the X(+)/potassium ATPases subunit beta family.
Cellular localization

Membrane.
Target information above from: UniProt accession P05026 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 481 Human
- Entrez Gene: 11931 Mouse
- Entrez Gene: 25650 Rat
- Omim: 182330 Human
- SwissProt: P05026 Human
- SwissProt: P14094 Mouse
- SwissProt: P07340 Rat
- Unigene: 291196 Human
- Unigene: 4550 Mouse
- Unigene: 8925 Rat
see all
Alternative names
- Adenosinetriphosphatase antibody
- AT1B1_HUMAN antibody
- ATP 1B antibody
- ATP1B antibody
- ATP1B1 antibody
- ATPase Na+/K+ transporting beta 1 polypeptide antibody
- Atpb 1 antibody
- ATPBS antibody
- Beta 1 subunit of Na(+) K(+) ATPase antibody
- Na K ATPase beta 1 polypeptide antibody
- Na+/K+ ATPase beta 1 subunit antibody
- Sodium/potassium dependent ATPase beta 1 subunit antibody
- Sodium/potassium transporting ATPase beta 1 chain antibody
- Sodium/potassium transporting ATPase subunit beta 1 antibody
- Sodium/potassium-dependent ATPase subunit beta-1 antibody
- Sodium/potassium-transporting ATPase subunit beta-1 antibody
see all

Western blot - Anti-ATP1B1 antibody [EPR12195] (ab193669)

Anti-ATP1B1 antibody [EPR12195] (ab193669) at 1/10000 dilution + Human fetal brain lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 35 kDa
Observed band size: 45-50 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-ATP1B1 antibody [EPR12195] (ab193669)

All lanes : Anti-ATP1B1 antibody [EPR12195] (ab193669) at 1/1000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 35 kDa
Observed band size: 45-50 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-ATP1B1 antibody [EPR12195] (ab193669)

All lanes : Anti-ATP1B1 antibody [EPR12195] (ab193669) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 35 kDa
Observed band size: 45-50 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] (ab193669)

Immunohistochemical analysis of paraffin-embedded Human renal adenocarcinoma tissue labeling ATP1B1 with ab193669 at 1/100 dilution (7.8μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] (ab193669)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ATP1B1 with ab193669 at 1/100 dilution (7.8μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] (ab193669)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ATP1B1 with ab193669 at 1/100 dilution (7.8μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot protocols
Immunohistochemistry protocols

Datasheet

ab193669 has not yet been referenced specifically in any publications.

Publishing research using ab193669? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Recombinant Anti-ATP1B1 antibody [EPR12195] (ab193669)

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

Applications

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As