Recombinant Anti-ATP1B2 antibody [EPR15461(B)] - BSA and Azide free (ab240290)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15461(B)] to ATP1B2 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ATP1B2 antibody [EPR15461(B)] - BSA and Azide free
See all ATP1B2 primary antibodies -
Description
Rabbit monoclonal [EPR15461(B)] to ATP1B2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human cerebellum lysate.
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General notes
ab240290 is the carrier-free version of ab185207.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15461(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 45 - 50 kDa (predicted molecular weight: 33 kDa).
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 45 - 50 kDa (predicted molecular weight: 33 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Function
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known. -
Sequence similarities
Belongs to the X(+)/potassium ATPases subunit beta family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 482 Human
- Entrez Gene: 11932 Mouse
- Entrez Gene: 24214 Rat
- Omim: 182331 Human
- SwissProt: P14415 Human
- SwissProt: P14231 Mouse
- SwissProt: P13638 Rat
- Unigene: 643540 Human
see all -
Alternative names
- adhesion molecule on glia antibody
- AMOG antibody
- AT1B2 antibody
see all
Images
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Western blot analysis of ATP1B2 in immunoprecipitation pellets from Human Y79 lysate. Lane 1 ab185207 used at 1/50 dilution. Lane 2 PBS negative control Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185207).
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Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207) at 1/20000 dilution + Human cerebellum lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 33 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185207).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab240290 has not yet been referenced specifically in any publications.