Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15461(B)] to ATP1B2 - C-terminal
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-ATP1B2 antibody [EPR15461(B)] - C-terminal
See all ATP1B2 primary antibodies
DescriptionRabbit monoclonal [EPR15461(B)] to ATP1B2 - C-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- Y79 and Human cerebellum lysates.
This product was previously labelled as ATPase Na+/ K+ beta 2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab185207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/50 - 1/80.|
|WB||1/10000 - 1/50000. Detects a band of approximately 45-50 kDa (predicted molecular weight: 33 kDa).|
FunctionThis is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known.
Sequence similaritiesBelongs to the X(+)/potassium ATPases subunit beta family.
- Information by UniProt
- adhesion molecule on glia antibody
- AMOG antibody
- AT1B2 antibody
Western blot analysis of ATP1B2 in immunoprecipitation pellets from Human Y79 lysate. Lane 1 ab185207 used at 1/50 dilution. Lane 2 PBS negative control Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.
Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207) at 1/20000 dilution + Human cerebellum lysate at 10 µg
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 33 kDa
Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207) at 1/20000 dilution + Y79 lysate at 20 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa
ab185207 has been referenced in 1 publication.
- Batiuk MY et al. An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody. J Biol Chem 292:8874-8891 (2017). PubMed: 28373281