Recombinant
RabMAb

Recombinant Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207)

Overview

  • Product name

    Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal
    See all ATP1B2 primary antibodies
  • Description

    Rabbit monoclonal [EPR15461(B)] to ATP1B2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human ATP1B2 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P14415
    (Peptide available as ab205972)

  • Positive control

    • Y79 and Human cerebellum lysates.
  • General notes

     

     

     This product was previously labelled as ATPase Na+/ K+ beta 2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15461(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab185207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50 - 1/80.
WB 1/10000 - 1/50000. Detects a band of approximately 45-50 kDa (predicted molecular weight: 33 kDa).

Target

  • Function

    This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known.
  • Sequence similarities

    Belongs to the X(+)/potassium ATPases subunit beta family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • adhesion molecule on glia antibody
    • AMOG antibody
    • AT1B2 antibody
    • AT1B2_HUMAN antibody
    • ATP1B2 antibody
    • ATPase Na+/K+ transporting beta 2 polypeptide antibody
    • ATPB2 antibody
    • ATPB2S antibody
    • MGC93648 antibody
    • Na+/K+ -ATPase beta 2 subunit antibody
    • Na, K ATPase beta 2 polypeptide antibody
    • RATATPB2S antibody
    • sodium potassium ATPase subunit beta 2 (non-catalytic) antibody
    • sodium pump subunit beta 2 antibody
    • sodium/potassium dependent ATPase beta 2 subunit antibody
    • Sodium/potassium dependent ATPase subunit beta 2 antibody
    • sodium/potassium transporting ATPase beta 2 chain antibody
    • sodium/potassium transporting ATPase subunit beta 2 antibody
    • Sodium/potassium-dependent ATPase subunit beta-2 antibody
    • Sodium/potassium-transporting ATPase subunit beta-2 antibody
    see all

Images

  • Western blot analysis of ATP1B2 in immunoprecipitation pellets from Human Y79 lysate. Lane 1 ab185207 used at 1/50 dilution. Lane 2 PBS negative control Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.

  • Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207) at 1/20000 dilution + Human cerebellum lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 33 kDa

  • Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207) at 1/20000 dilution + Y79 lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa

References

This product has been referenced in:

  • Batiuk MY  et al. An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody. J Biol Chem 292:8874-8891 (2017). Read more (PubMed: 28373281) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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