Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15461(B)] to ATP1B2 - C-terminal
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-ATP1B2 antibody [EPR15461(B)] - C-terminal
See all ATP1B2 primary antibodies
DescriptionRabbit monoclonal [EPR15461(B)] to ATP1B2 - C-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- Y79 and Human cerebellum lysates.
This product was previously labelled as ATPase Na+/ K+ beta 2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab185207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/50 - 1/80.|
|WB||1/10000 - 1/50000. Detects a band of approximately 45-50 kDa (predicted molecular weight: 33 kDa).|
FunctionThis is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known.
Sequence similaritiesBelongs to the X(+)/potassium ATPases subunit beta family.
- Information by UniProt
- adhesion molecule on glia antibody
- AMOG antibody
- AT1B2 antibody
Western blot analysis of ATP1B2 in immunoprecipitation pellets from Human Y79 lysate. Lane 1 ab185207 used at 1/50 dilution. Lane 2 PBS negative control Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.
Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207) at 1/20000 dilution + Human cerebellum lysate at 10 µg
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 33 kDa
Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207) at 1/20000 dilution + Y79 lysate at 20 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa
ab185207 has been referenced in 1 publication.
- Batiuk MY et al. An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody. J Biol Chem 292:8874-8891 (2017). PubMed: 28373281