RecombinantRabMAb

Recombinant Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207)

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Immunoprecipitation - Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207)
  • Western blot - Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207)
  • Western blot - Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207)
  • Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15461(B)] to ATP1B2 - C-terminal
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal
    See all ATP1B2 primary antibodies

  • Description

    Rabbit monoclonal [EPR15461(B)] to ATP1B2 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    within Human ATP1B2 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P14415
    (Peptide available as ab205972)

  • Positive control

    • Y79 and Human cerebellum lysates.

  • General notes

     

     

     This product was previously labelled as ATPase Na+/ K+ beta 2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15461(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab185207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50 - 1/80.
WB 1/10000 - 1/50000. Detects a band of approximately 45-50 kDa (predicted molecular weight: 33 kDa).

Target

  • Function

    This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known.

  • Sequence similarities

    Belongs to the X(+)/potassium ATPases subunit beta family.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • adhesion molecule on glia antibody
    • AMOG antibody
    • AT1B2 antibody
    • AT1B2_HUMAN antibody
    • ATP1B2 antibody
    • ATPase Na+/K+ transporting beta 2 polypeptide antibody
    • ATPB2 antibody
    • ATPB2S antibody
    • MGC93648 antibody
    • Na+/K+ -ATPase beta 2 subunit antibody
    • Na, K ATPase beta 2 polypeptide antibody
    • RATATPB2S antibody
    • sodium potassium ATPase subunit beta 2 (non-catalytic) antibody
    • sodium pump subunit beta 2 antibody
    • sodium/potassium dependent ATPase beta 2 subunit antibody
    • Sodium/potassium dependent ATPase subunit beta 2 antibody
    • sodium/potassium transporting ATPase beta 2 chain antibody
    • sodium/potassium transporting ATPase subunit beta 2 antibody
    • Sodium/potassium-dependent ATPase subunit beta-2 antibody
    • Sodium/potassium-transporting ATPase subunit beta-2 antibody
    see all

Images

  • Immunoprecipitation - Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207)
    Immunoprecipitation - Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207)

    Western blot analysis of ATP1B2 in immunoprecipitation pellets from Human Y79 lysate. Lane 1 ab185207 used at 1/50 dilution. Lane 2 PBS negative control Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.

  • Western blot - Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207)
    Western blot - Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207)
    Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207) at 1/20000 dilution + Human cerebellum lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 33 kDa

  • Western blot - Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207)
    Western blot - Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207)
    Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207) at 1/20000 dilution + Y79 lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa

  • Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207)
    Anti-ATP1B2 antibody [EPR15461(B)] - C-terminal (ab185207)

References (1)

Publishing research using ab185207? Please let us know so that we can cite the reference in this datasheet.

ab185207 has been referenced in 1 publication.

  • Batiuk MY  et al. An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody. J Biol Chem 292:8874-8891 (2017). PubMed: 28373281

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